XX
 XX
 XX
 FA Bach1
 XX
 SY BTB and CNC homolog
1; transcription regulator protein BACH1.
 XX
 OS mouse,
Mus musculus
 OC eukaryota; animalia; metazoa; chordata; vertebrata;
tetrapoda; mammalia;
 OC eutheria; rodentia; myomorpha; muridae;
murinae
 XX
 XX
 CL C0008; bZIP.
 XX
 SZ 739 AA;
81.4 kDa (calc)
 XX
 XX
 SC translated from EMBL #D86603

XX
 FT 1 117 BTB domain [2].
 FT 24 130 PF00651; BTB.

FT 34 130 SM00225; BTB.
 FT 224 225 CP motif (dipeptide of
C and P).
 FT 301 302 CP motif (dipeptide of C and P).

FT 438 440 CP motif (dipeptide of C and P).
 FT 464 465 CP
motif (dipeptide of C and P).
 FT 495 496 CP motif (dipeptide
of C and P).
 FT 529 624 leucine zipper (CNC bZIP region) [2].

FT 556 622 SM00338; BRLZ.
 FT 561 622 PF00170; bZIP.
 FT
649 650 CP motif (dipeptide of C and P).
 XX
 SF 38% amino
acid identity to Bach2  [2];
 SF contains a BTB (Broad
complex-Tramtrack-Bric-a-brac) domain and a CNC
 SF (Cap�n�collar)
domain [2];
 SF bZIP (basic leucine zipper) is of CNC-type
which is characterized by an
 SF additional conserved region
preceding bZIP domain [2];
 SF BTB domain mediates (weak) homophilic
interaction within cells [2] and is
 SF important for (homo-)oligomer
formation [1];
 SF BTB domain also seems to allow Bach1/MafK
 heterodimers to
 SF interact with each other
[1];
 SF obviously expressed from the earliest phase of hematopoietic
cell
 SF development onwards [1];
 SF binds porphyrin hemin
(Kd of 140 nM) and obviously also heme [5];
 SF binding to
hemin (heme) abolished if CP motifs (dipeptide of C and P) no.

SF 3-6 act deleted (act together in a cooperative manner) [5];

SF hemin (heme) binding abolishes DNA-binding (e.g. heterodimer

SF  to site ) independent of presence of
BTB-domain
 SF [5];
 SF heme obviously does not interfere
with heterodimer formation with MafK
 SF T01435 [5];
 SF
hemin (heme) abolishes transcriptional repressor activity obviously
via
 SF binding to the CP motifs [5];
 XX
 CP (embryo:)
expressed at embryonic day 13 and probably day 10 and 17 [2];

CP expressed in fetal liver at embryonic day 13.5 [1]; (adult:)
high levels in
 CP intestine [2]; expressed in liver [2], bone
marrow, thymus [1]; lower
 CP levels in brain, spleen [2];
(adult cell types:) expressed in fractions of
 CP Ter-119,
Mac-1 (mono-macrophage), Gr-1 (granulocyte), B220 (B cells),

CP CD4/8-double positive cells (Tcells) [1]; (cell lines:) expressed
in C1300
 CP (neuroblastoma), NIH 3T3, P815 (mastocytoma),
FDCP-1 (bipotential myeloid
 CP progenitor) M1 (myelomonocytic
leukemic) MEL (murine erythroleukemia),
 CP BW5147 cell lines
[2]
 EX bone marrow,,,adult;high; northern blot; total RNA;
[1].
 EX brain,,,Theiler Stage 27;detectable; northern blot;
mRNA (poly-A); [2].
 EX brain,,,adult;detectable; northern
blot; mRNA (poly-A); [2].
 EX brain,,,adult;low; northern blot;
total RNA; [1].
 EX heart,,,adult;detectable; northern blot;
mRNA (poly-A); [2].
 EX kidney (right and left),,,adult;detectable;
northern blot; mRNA (poly-A);
 EX [2].
 EX liver,,,Theiler
Stage 21;very high; northern blot; total RNA; [1].
 EX liver,,,Theiler
Stage 25;very low; northern blot; mRNA (poly-A); [2].
 EX liver,,,adult;detectable;
northern blot; mRNA (poly-A); [2].
 EX mouse, Mus musculus,,,Theiler
Stage 16;detectable; northern blot; mRNA
 EX (poly-A); [2].

EX mouse, Mus musculus,,,Theiler Stage 21;detectable; northern
blot; mRNA
 EX (poly-A); [2].
 EX mouse, Mus musculus,,,Theiler
Stage 25;detectable; northern blot; mRNA
 EX (poly-A); [2].

EX muscles,,,adult;detectable; northern blot; mRNA (poly-A); [2].

EX small intestine,,,adult;very high; northern blot; mRNA (poly-A);
[2].
 EX spleen,,,adult;medium; northern blot; mRNA (poly-A);
[2].
 EX spleen,,,adult;medium; northern blot; total RNA; [1].

EX thymus,,,adult;very high; northern blot; total RNA; [1].

XX
 FF may act as transcriptional repressor [2] and seems to
lack functional
 FF transcriptional activation activity under
certain conditions [1];
 FF transactivates transcription of
its own gene  via MARE-like
 FF sequences probably
not through direct binding to its promoter but through
 FF
inactivating another repressing factor [4];
 FF heterodimerization
with MafK  [2];
 FF binds to DNA (beta-globin promotor
) with MafK  in a
 FF cooperative manner [2];

FF binds also to DNA as monodimer [2];
 FF competes with NF-E2
p45 (compare to ) for binding to
 FF heterodimeric
partner MafK (compare to ) and to DNA (MARE

FF sequence) [1];
 XX
 IN T01435; MafK; mouse, Mus musculus.

XX
 XX
 XX
 XX
 RN [1]; RE0016917.
 RX MEDLINE;
98234368.
 RA Igarashi K., Hoshino H., Muto A., Suwabe N.,
Nishikawa S., Nakauchi H.,
 RA Yamamoto M.
 RT Multivalent
DNA binding complex generated by small Maf and Bach1 as a

RT possible biochemical basis for beta-globin locus control region
complex
 RL J. Biol. Chem. 273:11783-11790 (1998).
 RN [2];
RE0016914.
 RX MEDLINE; 97042438.
 RA Oyake T., Itoh K.,
Motohashi H., Hayashi N., Hoshino H., Nishizawa M.,
 RA Yamamoto
M., Igarashi K.
 RT Bach proteins belong to a novel family
of BTB-basic leucine zipper
 RT transcription factors that
interact with MafK and regulate transcription
 RT through the
NF-E2 site
 RL Mol. Cell. Biol. 16:6083-6095 (1996).
 RN
[3]; RE0022882.
 RX MEDLINE; 12356737.
 RA Sun J., Hoshino
H., Takaku K., Nakajima O., Muto A., Suzuki H., Tashiro S.,

RA Takahashi S., Shibahara S., Alam J., Taketo M. M., Yamamoto
M., Igarashi K.
 RT Hemoprotein Bach1 regulates enhancer availability
of heme oxygenase-1 gene.
 RL EMBO J. 21:5216-5224 (2002).

RN [4]; RE0016916.
 RX MEDLINE; 21421302.
 RA Sun J., Muto
A., Hoshino H., Kobayashi A., Nishimura S., Yamamoto M.,
 RA
Hayashi N., Ito E., Igarashi K.
 RT The Promoter of Mouse Transcription
Repressor bach1 Is Regulated by Sp1 and
 RT Trans-Activated
by Bach1
 RL J. Biochem. 130:385-392 (2001).
 RN [5]; RE0016931.

RX MEDLINE; 21280805.
 RA Ogawa K., Sun J., Taketani S., Nakajima
O., Nishitani C., Sassa S., Hayashi
 RA N., Yamamoto M., Shibahara
S., Fujita H., Igarashi K.
 RT Heme mediates derepression of
Maf recognition element through direct
 RT binding to transcription
repressor Bach1
 RL EMBO J. 20:2835-2843 (2001).
 XX