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Gene set: ESC_UP_BHATTACHARYA (C2:CLIN:0016)
Standard nameESC_UP_BHATTACHARYA
Systematic nameC2:CLIN:0016
Brief descriptionGenes upregulated in undifferentiated human embryonic stem cells.
CategoryC2: Curated
Sub-categoryCLIN: Clinical
Full description or Abstract
Human embryonic stem (huES) cells have the ability to differentiate
into a variety of cell lineages and potentially provide a source
of differentiated cells for many therapeutic uses. However, little
is known about the mechanism of differentiation of huES cells
and factors regulating cell development. We have used high-quality
microarrays containing 16 659 seventy-base pair oligonucleotides
to examine gene expression in 6 of the 11 available huES cell
lines. Expression was compared against pooled RNA from multiple
tissues (universal RNA) and genes enriched in huES cells were
identified. All 6 cell lines expressed multiple markers of the
undifferentiated state and shared significant homology in gene
expression (overall similarity coefficient > 0.85).A common subset
of 92 genes was identified that included Nanog, GTCM-1, connexin
43 (GJA1), oct-4, and TDGF1 (cripto). Gene expression was confirmed
by a variety of techniques including comparison with databases,
reverse transcriptase-polymerase chain reaction, focused cDNA
microarrays, and immunocytochemistry. Comparison with published
"stemness" genes revealed a limited overlap, suggesting little
similarity with other stem cell populations. Several novel ES
cell-specific expressed sequence tags were identified and mapped
to the human genome. These results represent the first detailed
characterization of undifferentiated huES cells and provide a
unique set of markers to profile and better understand the biology
of huES cells. 
Publication URL15070671
External linksna
Keywords & MeSH headingsComputational Biology,Cell Differentiation/genetics,Cell Line,Gene Expression Profiling,Oligonucleotide Array Sequence Analysis,Comparative Study,P.H.S.,Immunohistochemistry,Humans,Stem Cells,Gene Expression,Reverse Transcriptase Polymerase Chain Reaction,Mice,cytology,Genetic Markers,Expressed Sequence Tags,metabolism
OrganismHuman
Contributed byKate Stafford
Source platformGene_Symbol
Downloadgrp|xml|map
Genes

92 genes, 92 accessions (Toggle view)

GeneTitleLinks
ARL8ADP-ribosylation factor-like 8Source|GeneCards
ZNF257zinc finger protein 257Source|GeneCards
KRT18keratin 18Source|GeneCards
MTHFD2methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolaseSource|GeneCards
ZNF43zinc finger protein 43 (HTF6)Source|GeneCards
SNRPFsmall nuclear ribonucleoprotein polypeptide FSource|GeneCards
SLC16A1solute carrier family 16 (monocarboxylic acid transporters), member 1Source|GeneCards
RPS24ribosomal protein S24Source|GeneCards
HDAC2histone deacetylase 2Source|GeneCards
DSG2desmoglein 2Source|GeneCards
IMP-2IGF-II mRNA-binding protein 2Source|GeneCards
MGC27165hypothetical protein MGC27165Source|GeneCards
TNNT1troponin T1, skeletal, slowSource|GeneCards
MAD2L2MAD2 mitotic arrest deficient-like 2 (yeast)Source|GeneCards
RPL4ribosomal protein L4Source|GeneCards
MGST1microsomal glutathione S-transferase 1Source|GeneCards
RPLP0ribosomal protein, large, P0Source|GeneCards
FABP5fatty acid binding protein 5 (psoriasis-associated)Source|GeneCards
PITX2paired-like homeodomain transcription factor 2Source|GeneCards
ELOVL6ELOVL family member 6, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast)Source|GeneCards
GJA1gap junction protein, alpha 1, 43kDa (connexin 43)Source|GeneCards
HSPA4heat shock 70kDa protein 4Source|GeneCards
GDF3growth differentiation factor 3Source|GeneCards
SERPINH1serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)Source|GeneCards
LRRN1leucine rich repeat neuronal 1Source|GeneCards
ZNF117zinc finger protein 117 (HPF9)Source|GeneCards
HNRPABheterogeneous nuclear ribonucleoprotein A/BSource|GeneCards
SMSspermine synthaseSource|GeneCards
KPNA2karyopherin alpha 2 (RAG cohort 1, importin alpha 1)Source|GeneCards
PPATphosphoribosyl pyrophosphate amidotransferaseSource|GeneCards
ACTCactin, alpha, cardiac muscleSource|GeneCards
KRT8keratin 8Source|GeneCards
C20ORF129chromosome 20 open reading frame 129Source|GeneCards
TDGF1Teratocarcinoma-derived growth factor 1Source|GeneCards
NBR2neighbor of BRCA1 gene 2Source|GeneCards
RPL7ribosomal protein L7Source|GeneCards
PSIP1PC4 and SFRS1 interacting protein 1Source|GeneCards
NASPnuclear autoantigenic sperm protein (histone-binding)Source|GeneCards
CRABP2cellular retinoic acid binding protein 2Source|GeneCards
TUBB-5tubulin beta-5Source|GeneCards
CYP26A1cytochrome P450, family 26, subfamily A, polypeptide 1Source|GeneCards
RPL6ribosomal protein L6Source|GeneCards
CCT8chaperonin containing TCP1, subunit 8 (theta)Source|GeneCards
GALgalaninSource|GeneCards
CRABP1Cellular retinoic acid binding protein 1Source|GeneCards
NME2non-metastatic cells 2, protein (NM23B) expressed inSource|GeneCards
EPRSglutamyl-prolyl-tRNA synthetaseSource|GeneCards
LDHBlactate dehydrogenase BSource|GeneCards
GSH1GS homeobox 1Source|GeneCards
LAPTM4Blysosomal associated protein transmembrane 4 betaSource|GeneCards
CALUcalumeninSource|GeneCards
MTHFD1methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetaseSource|GeneCards
CCNCcyclin CSource|GeneCards
HMGB2high-mobility group box 2Source|GeneCards
SSBSjogren syndrome antigen B (autoantigen La)Source|GeneCards
C15ORF15chromosome 15 open reading frame 15Source|GeneCards
CDC2cell division cycle 2, G1 to S and G2 to MSource|GeneCards
PODXLpodocalyxin-likeSource|GeneCards
EIF4A1eukaryotic translation initiation factor 4A, isoform 1Source|GeneCards
PSMA2proteasome (prosome, macropain) subunit, alpha type, 2Source|GeneCards
SFRP2secreted frizzled-related protein 2Source|GeneCards
IDH1isocitrate dehydrogenase 1 (NADP+), solubleSource|GeneCards
SETSET translocation (myeloid leukemia-associated)Source|GeneCards
CCNB1cyclin B1Source|GeneCards
PSMA3proteasome (prosome, macropain) subunit, alpha type, 3Source|GeneCards
TK1thymidine kinase 1, solubleSource|GeneCards
DDX21DEAD (Asp-Glu-Ala-Asp) box polypeptide 21Source|GeneCards
PTTG1pituitary tumor-transforming 1Source|GeneCards
RPL24ribosomal protein L24Source|GeneCards
KIF4Akinesin family member 4ASource|GeneCards
IMPDH2IMP (inosine monophosphate) dehydrogenase 2Source|GeneCards
SEMA6Asema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6ASource|GeneCards
NPM1nucleophosmin (nucleolar phosphoprotein B23, numatrin)Source|GeneCards


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