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Gene set: GNATENKO_PLATELET (C2:PERT:0070)
Standard nameGNATENKO_PLATELET
Systematic nameC2:PERT:0070
Brief descriptionTop expressed genes in human platelet cells.
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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Human platelets are anucleate blood cells that retain cytoplasmic
mRNA and maintain functionally intact protein translational capabilities.
We have adapted complementary techniques of microarray and serial
analysis of gene expression (SAGE) for genetic profiling of highly
purified human blood platelets. Microarray analysis using the
Affymetrix HG-U95Av2 approximately 12 600-probe set maximally
identified the expression of 2147 (range, 13%-17%) platelet-expressed
transcripts, with approximately 22% collectively involved in metabolism
and receptor/signaling, and an overrepresentation of genes with
unassigned function (32%). In contrast, a modified SAGE protocol
using the Type IIS restriction enzyme MmeI (generating 21-base
pair [bp] or 22-bp tags) demonstrated that 89% of tags represented
mitochondrial (mt) transcripts (enriched in 16S and 12S ribosomal
RNAs), presumably related to persistent mt-transcription in the
absence of nuclear-derived transcripts. The frequency of non-mt
SAGE tags paralleled average difference values (relative expression)
for the most "abundant" transcripts as determined by microarray
analysis, establishing the concordance of both techniques for
platelet profiling. Quantitative reverse transcription-polymerase
chain reaction (PCR) confirmed the highest frequency of mt-derived
transcripts, along with the mRNAs for neurogranin (NGN, a protein
kinase C substrate) and the complement lysis inhibitor clusterin
among the top 5 most abundant transcripts. For confirmatory characterization,
immunoblots and flow cytometric analyses were performed, establishing
abundant cell-surface expression of clusterin and intracellular
expression of NGN. These observations demonstrate a strong correlation
between high transcript abundance and protein expression, and
they establish the validity of transcript analysis as a tool for
identifying novel platelet proteins that may regulate normal and
pathologic platelet (and/or megakaryocyte) functions.
Publication URL12433680
External linksna
Keywords & MeSH headingsblood,Glycoproteins/blood/genetics,Calmodulin-Binding Proteins/blood/genetics,RNA,Nerve Tissue Proteins/blood/genetics,Blood Platelets,Non-P.H.S.,Oligonucleotide Array Sequence Analysis,Gene Expression Profiling,P.H.S.,Mitochondria/chemistry,Messenger,Cell Separation,Deoxyribonucleases,Humans,chemistry,Molecular Chaperones/blood/genetics,Type II Site-Specific/metabolism,Reverse Transcriptase Polymerase Chain Reaction,Gene Library,metabolism
OrganismHuman
Contributed byKate Stafford
Source platformSeq_Accession
Downloadgrp|xml|map
Genes

46 genes, 50 accessions (Toggle view)

AccessionGeneTitleLinks
J03040SPARCsecreted protein, acidic, cysteine-rich (osteonectin)Source|GeneCards
Z83738HIST1H2BMhistone 1, H2bmSource|GeneCards
X00351ACTBactin, betaSource|GeneCards
X57985HIST2H2BEhistone 2, H2beSource|GeneCards
AA524802
J00153HBA2hemoglobin, alpha 2Source|GeneCards
M16279CD99CD99 antigenSource|GeneCards
M26252PKM2pyruvate kinase, muscleSource|GeneCards
M21121CCL5chemokine (C-C motif) ligand 5Source|GeneCards
AF070585TOP3Btopoisomerase (DNA) III betaSource|GeneCards
AB021288B2Mbeta-2-microglobulinSource|GeneCards
M17733TMSB4Xthymosin, beta 4, X-linkedSource|GeneCards
X54304MRCL3myosin regulatory light chain MRCL3Source|GeneCards
U59632GP1BBglycoprotein Ib (platelet), beta polypeptideSource|GeneCards
AB028950TLN1talin 1Source|GeneCards
AI540958
X77548NCOA4nuclear receptor coactivator 4Source|GeneCards
X56009GNASGNAS complex locusSource|GeneCards
Z12962RPL41ribosomal protein L41Source|GeneCards
Y12711PGRMC1progesterone receptor membrane component 1Source|GeneCards
AB009010UBCubiquitin CSource|GeneCards
J04755FTHP1ferritin, heavy polypeptide pseudogene 1Source|GeneCards
AL050396FLNAfilamin A, alpha (actin binding protein 280)Source|GeneCards
M25897PF4platelet factor 4 (chemokine (C-X-C motif) ligand 4)Source|GeneCards
L40399MLH3mutL homolog 3 (E. coli)Source|GeneCards
M11353H3F3AH3 histone, family 3ASource|GeneCards
X13710GPX1glutathione peroxidase 1Source|GeneCards
AL031670RNF24ring finger protein 24Source|GeneCards
U34995GAPDglyceraldehyde-3-phosphate dehydrogenaseSource|GeneCards
X95404CFL1cofilin 1 (non-muscle)Source|GeneCards
X58536HLA-Cmajor histocompatibility complex, class I, CSource|GeneCards
X99076NRGNneurogranin (protein kinase C substrate, RC3)Source|GeneCards
M22919MYL6myosin, light polypeptide 6, alkali, smooth muscle and non-muscleSource|GeneCards
AF045229RGS10regulator of G-protein signalling 10Source|GeneCards
X06956TUBA1tubulin, alpha 1 (testis specific)Source|GeneCards
X04409GNASGNAS complex locusSource|GeneCards
D78361OAZ1ornithine decarboxylase antizyme 1Source|GeneCards
L20941FTH1ferritin, heavy polypeptide 1Source|GeneCards
X56841HLA-Emajor histocompatibility complex, class I, ESource|GeneCards
D78577YWHAHtyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptideSource|GeneCards
U90551HIST1H2AChistone 1, H2acSource|GeneCards
M25915CLUclusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)Source|GeneCards
M34480ITGA2Bintegrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41B)Source|GeneCards
D21261TAGLN2transgelin 2Source|GeneCards
M54995PPBPpro-platelet basic protein (chemokine (C-X-C motif) ligand 7)Source|GeneCards
M25079HBB /// HBDhemoglobin, beta /// hemoglobin, deltaSource|GeneCards
M14630PTMAprothymosin, alpha (gene sequence 28)Source|GeneCards
L19779HIST2H2AAhistone 2, H2aaSource|GeneCards
M14539F13A1coagulation factor XIII, A1 polypeptideSource|GeneCards
AA477898

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