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Gene set: IL5_UP_BYSTROM (C2:PERT:0062)
Standard nameIL5_UP_BYSTROM
Systematic nameC2:PERT:0062
Brief descriptionGenes downregulated in mouse bone marrow in response to interleukin-5.
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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Interleukin-5 (IL-5) is a hematopoietic differentiation factor
that promotes the development of mature eosinophils from progenitors
in bone marrow. We present a multifactorial microarray study documenting
the transcriptional events in bone marrow of wild-type and IL-5-deficient
mice at baseline and in response to infection with Schistosoma
mansoni. The microarray data were analyzed by a 4-way subtractive
algorithm that eliminated confounding non-IL-5-related sequelae
of schistosome infection as well as alterations in gene expression
among uninfected mice. Among the most prominent findings, we observed
7- to 40-fold increased expression of transcripts encoding the
classic eosinophil granule proteins (eosinophil peroxidase, major
basic protein, the ribonucleases) together with arachidonate-15-lipoxygenase
and protease inhibitor plasminogen activator inhibitor 2 (PAI-2),
in the IL-5-producing, infected wild-type mice only. This was
accompanied by increased transcription of genes involved in secretory
protein biosynthesis and granule-vesicle formation. Interestingly,
we did not detect increased expression of genes encoding eosinophil-related
chemokine receptors (CCR1, CCR3) or members of the GATA or CCAAT/enhancer
binding protein (C/EBP) transcription factor families. These data
suggest that the IL-5-responsive progenitors in the mouse bone
marrow are already significantly committed to the eosinophil lineage
and that IL-5 promotes differentiation of these committed progenitors
into cells with recognizable and characteristic cytoplasmic granules
and granule proteins.
Publication URL14525773
External linksna
Keywords & MeSH headingsKnockout,Oligonucleotide Array Sequence Analysis,Gene Expression Profiling,P.H.S.,Blood Proteins/genetics,Complementary/genetics,immunology,Hematopoietic Stem Cells,genetics,Genetic,Mice,Hematopoiesis,deficiency,Schistosomiasis mansoni/genetics/immunology,metabolism,Eosinophilia/etiology,DNA,Base Sequence,Transcription,Inbred C57BL,Ribonucleases/genetics,Eosinophil Granule Proteins,Reverse Transcriptase Polymerase Chain Reaction,Interleukin-5,cytology
OrganismMouse
Contributed byKate Stafford
Source platformSeq_Accession
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Genes

55 genes, 55 accessions (Toggle view)

AccessionGeneTitleLinks
DNCHC1DNCH1dynein, cytoplasmic, heavy polypeptide 1Source|GeneCards
NUCB1NUCB1nucleobindin 1Source|GeneCards
IL11RA1
EAR3NR2F1nuclear receptor subfamily 2, group F, member 1Source|GeneCards
DOK2DOK2docking protein 2, 56kDaSource|GeneCards
AV347947
HDGFHDGFhepatoma-derived growth factor (high-mobility group protein 1-like)Source|GeneCards
HDAC2HDAC2histone deacetylase 2Source|GeneCards
ETF1ETF1eukaryotic translation termination factor 1Source|GeneCards
IL3RAIL3RAinterleukin 3 receptor, alpha (low affinity)Source|GeneCards
CBX3CBX3chromobox homolog 3 (HP1 gamma homolog, Drosophila)Source|GeneCards
GGA1GGA1golgi associated, gamma adaptin ear containing, ARF binding protein 1Source|GeneCards
SLC18A2SLC18A2solute carrier family 18 (vesicular monoamine), member 2Source|GeneCards
LYL1LYL1lymphoblastic leukemia derived sequence 1Source|GeneCards
CTDSP2CTDSP2CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2Source|GeneCards
9430080K19RIK
CAMPCAMPcathelicidin antimicrobial peptideSource|GeneCards
VAMP8VAMP8vesicle-associated membrane protein 8 (endobrevin)Source|GeneCards
RPL41RPL41ribosomal protein L41Source|GeneCards
EPXEPXeosinophil peroxidaseSource|GeneCards
EAR1NR1D1nuclear receptor subfamily 1, group D, member 1Source|GeneCards
TACSTD2TACSTD2tumor-associated calcium signal transducer 2Source|GeneCards
ALOX15ALOX15arachidonate 15-lipoxygenaseSource|GeneCards
ZFP35
PRG2PRG2proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)Source|GeneCards
GATA1GATA1GATA binding protein 1 (globin transcription factor 1)Source|GeneCards
AV105022
POLR2LPOLR2Lpolymerase (RNA) II (DNA directed) polypeptide L, 7.6kDaSource|GeneCards
GTF3C1GTF3C1general transcription factor IIIC, polypeptide 1, alpha 220kDaSource|GeneCards
GATA2GATA2GATA binding protein 2Source|GeneCards
PTPN2PTPN2protein tyrosine phosphatase, non-receptor type 2Source|GeneCards
CD63CD63CD63 antigen (melanoma 1 antigen)Source|GeneCards
AV055186
PRG3PRG3proteoglycan 3Source|GeneCards
BRD4BRD4bromodomain containing 4Source|GeneCards
STX3
VCAM1VCAM1vascular cell adhesion molecule 1Source|GeneCards
AF085809
GORASP2GORASP2golgi reassembly stacking protein 2, 55kDaSource|GeneCards
HIP1RHIP1Rhuntingtin interacting protein-1-relatedSource|GeneCards
RAF1RAF1v-raf-1 murine leukemia viral oncogene homolog 1Source|GeneCards
RBBP4RBBP4retinoblastoma binding protein 4Source|GeneCards
SMARCD2SMARCD2SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2Source|GeneCards
AV306387
NUCB2NUCB2nucleobindin 2Source|GeneCards
E2F1E2F1E2F transcription factor 1Source|GeneCards
PERPPERPPERP, TP53 apoptosis effectorSource|GeneCards
PHEMXPHEMXpan-hematopoietic expressionSource|GeneCards
LY6C
SERPINB2SERPINB2serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2Source|GeneCards
C530046L02RIK
NFKB1NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)Source|GeneCards
SETSETSET translocation (myeloid leukemia-associated)Source|GeneCards
RDBPRDBPRD RNA binding proteinSource|GeneCards
CTSHCTSHcathepsin HSource|GeneCards

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