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Gene set: PASSERINI_APOPTOSIS (C2:PERT:0079)
Standard namePASSERINI_APOPTOSIS
Systematic nameC2:PERT:0079
Brief descriptionGenes associated with cellular adhesion that are differentially expressed in endothelial cells of pig aortas from regions of disturbed flow (inner aortic arch) versus regions of undisturbed laminar flow (descending thoracic aorta).
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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In the arterial circulation, regions of disturbed flow (DF), which
are characterized by flow separation and transient vortices, are
susceptible to atherogenesis, whereas regions of undisturbed laminar
flow (UF) appear protected. Coordinated regulation of gene expression
by endothelial cells (EC) may result in differing regional phenotypes
that either favor or inhibit atherogenesis. Linearly amplified
RNA from freshly isolated EC of DF (inner aortic arch) and UF
(descending thoracic aorta) regions of normal adult pigs was used
to profile differential gene expression reflecting the steady
state in vivo. By using human cDNA arrays, approximately 2,000
putatively differentially expressed genes were identified through
false-discovery-rate statistical methods. A sampling of these
genes was validated by quantitative real-time PCR and/or immunostaining
en face. Biological pathway analysis revealed that in DF there
was up-regulation of several broad-acting inflammatory cytokines
and receptors, in addition to elements of the NF-kappaB system,
which is consistent with a proinflammatory phenotype. However,
the NF-kappaB complex was predominantly cytoplasmic (inactive)
in both regions, and no significant differences were observed
in the expression of key adhesion molecules for inflammatory cells
associated with early atherogenesis. Furthermore, there was no
histological evidence of inflammation. Protective profiles were
observed in DF regions, notably an enhanced antioxidative gene
expression. This study provides a public database of regional
EC gene expression in a normal animal, implicates hemodynamics
as a contributory mechanism to athero-susceptibility, and reveals
the coexistence of pro- and antiatherosclerotic transcript profiles
in susceptible regions. The introduction of additional risk factors
may shift this balance to favor lesion development.
Publication URL14983035
External linksna
Keywords & MeSH headingsComputational Biology,Vascular,Apoptosis/genetics,Male,Non-P.H.S.,Gene Expression Profiling,P.H.S.,Enzymes/genetics,Transcription,Polymerase Chain Reaction/methods,Endothelium,Swine,Regional Blood Flow,Proteins/genetics,genetics,Genetic,Gene Expression Regulation,physiology,Oxidation-Reduction,Aorta,Reproducibility of Results
OrganismPig
Contributed byKate Stafford
Source platformSeq_Accession
Downloadgrp|xml|map
Genes

40 genes, 43 accessions (Toggle view)

AccessionGeneTitleLinks
U13737CASP3caspase 3, apoptosis-related cysteine proteaseSource|GeneCards
AF032885FOXO1Aforkhead box O1A (rhabdomyosarcoma)Source|GeneCards
L08246MCL1myeloid cell leukemia sequence 1 (BCL2-related)Source|GeneCards
AF307851TP53tumor protein p53 (Li-Fraumeni syndrome)Source|GeneCards
Y15906API5apoptosis inhibitor 5Source|GeneCards
BC001599BIKBCL2-interacting killer (apoptosis-inducing)Source|GeneCards
BC008058PRKCZprotein kinase C, zetaSource|GeneCards
BC004983NFKBIAnuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alphaSource|GeneCards
U80017
X02851IL1Ainterleukin 1, alphaSource|GeneCards
D84476MAP3K5mitogen-activated protein kinase kinase kinase 5Source|GeneCards
L08850SNCAsynuclein, alpha (non A4 component of amyloid precursor)Source|GeneCards
X14474MAPTmicrotubule-associated protein tauSource|GeneCards
X52479PRKCAprotein kinase C, alphaSource|GeneCards
Y00630SERPINB2serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2Source|GeneCards
X76104DAPK1death-associated protein kinase 1Source|GeneCards
U08137FASLGFas ligand (TNF superfamily, member 6)Source|GeneCards
X93921DUSP7dual specificity phosphatase 7Source|GeneCards
X13334CD14CD14 antigenSource|GeneCards
M58603NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)Source|GeneCards
L06132VDAC1voltage-dependent anion channel 1Source|GeneCards
BC008308CUL4Acullin 4ASource|GeneCards
AF062536CUL1cullin 1Source|GeneCards
Y09397BCL2A1BCL2-related protein A1Source|GeneCards
BC001901BADBCL2-antagonist of cell deathSource|GeneCards
BC006203
BC008378PDCD2programmed cell death 2Source|GeneCards
U03106CDKN1Acyclin-dependent kinase inhibitor 1A (p21, Cip1)Source|GeneCards
M13994BCL2B-cell CLL/lymphoma 2Source|GeneCards
BC002523PTPN6protein tyrosine phosphatase, non-receptor type 6Source|GeneCards
X98173CASP8caspase 8, apoptosis-related cysteine proteaseSource|GeneCards
M37763NTF3neurotrophin 3Source|GeneCards
AF005058CXCR4chemokine (C-X-C motif) receptor 4Source|GeneCards
AF013956CBX4chromobox homolog 4 (Pc class homolog, Drosophila)Source|GeneCards
M60974GADD45Agrowth arrest and DNA-damage-inducible, alphaSource|GeneCards
AF087853GADD45Bgrowth arrest and DNA-damage-inducible, betaSource|GeneCards
M87507CASP1caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase)Source|GeneCards
AF070674BIRC3baculoviral IAP repeat-containing 3Source|GeneCards
L49207PTK2BPTK2B protein tyrosine kinase 2 betaSource|GeneCards
M22877CYCScytochrome c, somaticSource|GeneCards
AF173003BFARbifunctional apoptosis regulatorSource|GeneCards
U18321
BC001693LGALS1lectin, galactoside-binding, soluble, 1 (galectin 1)Source|GeneCards

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