[an error occurred while processing this directive]
Gene set: TENEDINI_MK (C2:PERT:0089)
Standard nameTENEDINI_MK
Systematic nameC2:PERT:0089
Brief descriptionGenes essential to the development of megakaryocytes, as expressed in normal cells and essential thrombocythemic cells (ET).
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract
Gene expression profiles of bone marrow (BM) CD34-derived megakaryocytic
cells (MKs) were compared in patients with essential thrombocythemia
(ET) and healthy subjects using oligonucleotide microarray analysis
to identify differentially expressed genes and disease-specific
transcripts. We found that proapoptotic genes such as BAX, BNIP3,
and BNIP3L were down-regulated in ET MKs together with genes that
are components of the mitochondrial permeability transition pore
complex, a system with a pivotal role in apoptosis. Conversely,
antiapoptotic genes such as IGF1-R and CFLAR were up-regulated
in the malignant cells, as was the SDF1 gene, which favors cell
survival. On the basis of the array results, we characterized
apoptosis of normal and ET MKs by time-course evaluation of annexin-V
and sub-G1 peak DNA stainings of immature and mature MKs after
culture in serum-free medium with an optimal thrombopoietin concentration,
and annexin-V-positive MKs only, with decreasing thrombopoietin
concentrations. ET MKs were more resistant to apoptosis than their
normal counterparts. We conclude that imbalance between proliferation
and apoptosis seems to be an important step in malignant ET megakaryocytopoiesis.
Publication URL15271793
External linksna
Keywords & MeSH headingsMale,CD34/metabolism,Middle Aged,Gene Expression Profiling,Female,Cells,Cell Lineage,Megakaryocytes,Humans,Hemorrhagic,Apoptosis,genetics,Antigens,cytology,physiology,Aged,Cultured,physiopathology,Thrombocythemia
OrganismHuman
Contributed byKate Stafford
Source platformSeq_Accession
Downloadgrp|xml|map
Genes

54 genes, 60 accessions (Toggle view)

AccessionGeneTitleLinks
U60521CASP9caspase 9, apoptosis-related cysteine proteaseSource|GeneCards
U66879BADBCL2-antagonist of cell deathSource|GeneCards
NM_002017
NM_005093CBFA2T2core-binding factor, runt domain, alpha subunit 2; translocated to, 2Source|GeneCards
AK022920PDGFBplatelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)Source|GeneCards
NM_002050GATA2GATA binding protein 2Source|GeneCards
NM_004346CASP3caspase 3, apoptosis-related cysteine proteaseSource|GeneCards
D87461BCL2L2BCL2-like 2Source|GeneCards
U65404KLF1Kruppel-like factor 1 (erythroid)Source|GeneCards
L13974NFE2nuclear factor (erythroid-derived 2), 45kDaSource|GeneCards
NM_000419ITGA2Bintegrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41B)Source|GeneCards
M21121CCL5chemokine (C-C motif) ligand 5Source|GeneCards
M30601GATA1GATA binding protein 1 (globin transcription factor 1)Source|GeneCards
R64130PPBPpro-platelet basic protein (chemokine (C-X-C motif) ligand 7)Source|GeneCards
AJ224869CXCR4chemokine (C-X-C motif) receptor 4Source|GeneCards
BC000125TGFB1transforming growth factor, beta 1 (Camurati-Engelmann disease)Source|GeneCards
M15330IL1Binterleukin 1, betaSource|GeneCards
M23612
NM_002607PDGFAplatelet-derived growth factor alpha polypeptideSource|GeneCards
NM_005825RASGRP2RAS guanyl releasing protein 2 (calcium and DAG-regulated)Source|GeneCards
NM_003152STAT5Asignal transducer and activator of transcription 5ASource|GeneCards
BE407516CCNB1cyclin B1Source|GeneCards
NM_005375MYBv-myb myeloblastosis viral oncogene homolog (avian)Source|GeneCards
NM_005354JUNDjun D proto-oncogeneSource|GeneCards
U19495CXCL12chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)Source|GeneCards
NM_001769CD9CD9 antigen (p24)Source|GeneCards
NM_004702CCNE2cyclin E2Source|GeneCards
NM_005187CBFA2T3core-binding factor, runt domain, alpha subunit 2; translocated to, 3Source|GeneCards
NM_021960MCL1myeloid cell leukemia sequence 1 (BCL2-related)Source|GeneCards
NM_005373MPLmyeloproliferative leukemia virus oncogeneSource|GeneCards
BC001971CDKN1Bcyclin-dependent kinase inhibitor 1B (p27, Kip1)Source|GeneCards
NM_001188
AF022375VEGFvascular endothelial growth factorSource|GeneCards
NM_001315MAPK14mitogen-activated protein kinase 14Source|GeneCards
BC000627STAT3signal transducer and activator of transcription 3 (acute-phase response factor)Source|GeneCards
NM_002359MAFGv-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)Source|GeneCards
NM_001511CXCL1chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)Source|GeneCards
NM_016205PDGFCplatelet derived growth factor CSource|GeneCards
NM_004972JAK2Janus kinase 2 (a protein tyrosine kinase)Source|GeneCards
NM_000174GP9glycoprotein IX (platelet)Source|GeneCards
NM_000584IL8interleukin 8Source|GeneCards
AL524035CDC2cell division cycle 2, G1 to S and G2 to MSource|GeneCards
NM_000565IL6Rinterleukin 6 receptorSource|GeneCards
AB043821GP6glycoprotein VI (platelet)Source|GeneCards
AI671049CCNE1cyclin E1Source|GeneCards
NM_000173GP1BAglycoprotein Ib (platelet), alpha polypeptideSource|GeneCards
NM_001760CCND3cyclin D3Source|GeneCards
NM_000600IL6interleukin 6 (interferon, beta 2)Source|GeneCards
BE327172
NM_003189TAL1T-cell acute lymphocytic leukemia 1Source|GeneCards
BG029530JARID2Jumonji, AT rich interactive domain 2Source|GeneCards
AI189839ITGB3integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)Source|GeneCards
M97935STAT1signal transducer and activator of transcription 1, 91kDaSource|GeneCards
D38081TBXA2Rthromboxane A2 receptorSource|GeneCards
NM_002619PF4platelet factor 4 (chemokine (C-X-C motif) ligand 4)Source|GeneCards
NM_000576IL1Binterleukin 1, betaSource|GeneCards
NM_000222KITv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homologSource|GeneCards
NM_002467
M68520CDK2cyclin-dependent kinase 2Source|GeneCards
NM_000633BCL2B-cell CLL/lymphoma 2Source|GeneCards


[an error occurred while processing this directive]