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Gene set: ZHAN_TONSIL_PCBC (C2:PERT:0112)
Standard nameZHAN_TONSIL_PCBC
Systematic nameC2:PERT:0112
Brief descriptionTop 50 genes differentially expressed in CD19-enriched tonsil B cells and CD138-enriched tonsil plamsa cells.
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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To identify genes linked to normal plasma cell (PC) differentiation
and to classify multiple myeloma (MM) with respect to the expression
patterns of these genes, we analyzed global mRNA expression in
CD19-enriched B cells (BCs) from 7 tonsils, CD138-enriched PCs
from 11 tonsils, 31 normal bone marrow samples, and 74 MM bone
marrow samples using microarrays interrogating 6800 genes. Hierarchical
clustering analyses with 3288 genes clearly segregated the 4 cell
types, and chi-square and Wilcoxin rank sum tests (P <.0005) identified
359 and 500 previously defined and novel genes that distinguish
tonsil BCs from tonsil PCs (early differentiation genes [EDGs]),
and tonsil PCs from bone marrow PCs (late differentiation genes
[LDGs]), respectively. MM as a whole was found to have dramatically
variable expression of EDGs and LDGs, and one-way analysis of
variance (ANOVA) was used to identify the most variable EDGs (vEDGs)
and LDGs (v1LDG and v2LDG). Hierarchical cluster analysis with
these genes revealed that previously defined MM gene expression
subgroups (MM1-MM4) could be linked to one of the 3 normal cell
types. Clustering with 30 vEDGs revealed that 13 of 18 MM4 cases
clustered with tonsil BCs (P =.000 05), whereas 14 of 15 MM3 cases
clustered with tonsil PCs when using 50 v1LDG (P =.000 008), and
14 of 20 MM2 cases clustered with bone marrow PCs when using 50
v2LDG (P =.000 09). MM1 showed no significant linkage with normal
cell types studied. Thus, genes whose expression is linked to
distinct transitions in late-stage B-cell differentiation can
be used to classify MM. 
Publication URL12393520
External linksna
Keywords & MeSH headingsMembrane Glycoproteins,Tonsil/cytology,B-Lymphocytes/cytology/immunology,Cell Differentiation/genetics,RNA,Gene Expression Profiling,Plasma Cells,P.H.S.,Comparative Study,classification,Proteoglycans,Gene Expression Regulation/genetics,Multiple Myeloma,Humans,Messenger/analysis,pathology,cytology,Bone Marrow Cells,Cluster Analysis
OrganismHuman
Contributed byKate Stafford
Source platformGene_Symbol
Downloadgrp|xml|map
Genes

49 genes, 49 accessions (Toggle view)

GeneTitleLinks
EGR3early growth response 3Source|GeneCards
FOXM1forkhead box M1Source|GeneCards
STCHstress 70 protein chaperone, microsome-associated, 60kDaSource|GeneCards
HADHAhydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunitSource|GeneCards
IRF4interferon regulatory factor 4Source|GeneCards
ITGA6integrin, alpha 6Source|GeneCards
ETS1v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)Source|GeneCards
MYCV-myc myelocytomatosis viral oncogene homolog (avian)Source|GeneCards
TNFtumor necrosis factor (TNF superfamily, member 2)Source|GeneCards
SNRPCSmall nuclear ribonucleoprotein polypeptide CSource|GeneCards
ID3inhibitor of DNA binding 3, dominant negative helix-loop-helix proteinSource|GeneCards
HRASv-Ha-ras Harvey rat sarcoma viral oncogene homologSource|GeneCards
SPIBSpi-B transcription factor (Spi-1/PU.1 related)Source|GeneCards
VCLvinculinSource|GeneCards
LMNB1lamin B1Source|GeneCards
TERF2telomeric repeat binding factor 2Source|GeneCards
ELF1E74-like factor 1 (ets domain transcription factor)Source|GeneCards
BLKB lymphoid tyrosine kinaseSource|GeneCards
CCNG2cyclin G2Source|GeneCards
GBP1guanylate binding protein 1, interferon-inducible, 67kDaSource|GeneCards
MSH2mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)Source|GeneCards
LTBlymphotoxin beta (TNF superfamily, member 3)Source|GeneCards
SPI1spleen focus forming virus (SFFV) proviral integration oncogene spi1Source|GeneCards
BLR1Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)Source|GeneCards
RUNX3runt-related transcription factor 3Source|GeneCards
CIITAclass II, major histocompatibility complex, transactivatorSource|GeneCards
OGG18-oxoguanine DNA glycosylaseSource|GeneCards
CASP10caspase 10, apoptosis-related cysteine proteaseSource|GeneCards
RPA1replication protein A1, 70kDaSource|GeneCards
IL4Rinterleukin 4 receptorSource|GeneCards
CDC20CDC20 cell division cycle 20 homolog (S. cerevisiae)Source|GeneCards
SLC7A6solute carrier family 7 (cationic amino acid transporter, y+ system), member 6Source|GeneCards
TRAF5TNF receptor-associated factor 5Source|GeneCards
EEDembryonic ectoderm developmentSource|GeneCards
LIG1ligase I, DNA, ATP-dependentSource|GeneCards
CCNFcyclin FSource|GeneCards
ABCD3ATP-binding cassette, sub-family D (ALD), member 3Source|GeneCards
PECAM1platelet/endothelial cell adhesion molecule (CD31 antigen)Source|GeneCards
CSTF2cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDaSource|GeneCards
RELv-rel reticuloendotheliosis viral oncogene homolog (avian)Source|GeneCards
XBP1X-box binding protein 1Source|GeneCards
RAE1RAE1 RNA export 1 homolog (S. pombe)Source|GeneCards


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