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Gene set: 2ME2_CHAUHAN (C2:PERT:0063)
Standard name2ME2_CHAUHAN
Systematic nameC2:PERT:0063
Brief descriptionGenes whose expression is influenced by 2-methoxyestradiol (2ME2), a metabolite of estradiol that has antitumor activity, in multiple myeloma cells.
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract
Our previous study demonstrated that 2-methoxyestradiol (2ME2),
an estrogen derivative, induces apoptosis in multiple myeloma
(MM) cells; however, the related transcriptional events are unclear.
In the present study, we used oligonucleotide microarrays to identify
genes altered during 2ME2-induced apoptosis in MM cells. 2ME2
triggers an early transient induction of genes known to trigger
cell death and repression of growth/survival-related genes. Many
genes regulating cell defense/repair machinery also were transiently
induced. Since 2ME2 also induces apoptosis in MM cells resistant
to conventional therapies such as dexamethasone (Dex), we compared
the gene profiles of 2ME2-treated and Dex-resistant MM cells.
Our results suggest that 2ME2 overcomes Dex resistance by modulating
genes that confer chemoresistance in MM cells. Microarray results
were confirmed by Northern and Western blot analyses. A comparative
analysis of selected genes from freshly isolated MM patient cells
and 2ME2-treated MM.1S MM cells further provides an in vivo relevance
of our in vitro studies. Collectively, these findings suggest
genetic events mediating anti-MM activity of 2ME2, as well as
mechanisms whereby 2ME2 overcomes Dex resistance in MM cells.
These studies may therefore allow improved therapeutic use of
2ME2, based upon targeting genes that regulate MM cell growth
and survival.
Publication URL12480690
External linksna
Keywords & MeSH headingsNeoplastic,Heat-Shock Proteins/biosynthesis/genetics,Oligonucleotide Array Sequence Analysis,Gene Expression Profiling,Comparative Study,P.H.S.,Transfection,Neoplasm Proteins,Dexamethasone/pharmacology,Endoplasmic Reticulum/metabolism,Multiple Myeloma,Tumor Cells,Cysteine Endopeptidases/metabolism,Multienzyme Complexes/metabolism,Messenger/biosynthesis/genetics,analogs & derivatives,Reporter,Genes,Transcription Factors/biosynthesis/genetics,genetics,Ubiquitin/metabolism,Neoplasm,Estradiol,Cytoskeletal Proteins/biosynthesis/genetics,Protein Folding,Cultured/drug effects/metabolism,Proteasome Endopeptidase Complex,RNA,Non-P.H.S.,pharmacology,Neoplasm/biosynthesis/genetics,Interleukin-6/pharmacology,drug effects,pathology,Gene Expression Regulation,Drug Resistance,Subtraction Technique,biosynthesis,DNA-Binding Proteins/biosynthesis/genetics
OrganismHuman
Contributed byKate Stafford
Source platformSeq_Accession
Downloadgrp|xml|map
Genes

37 genes, 55 accessions (Toggle view)

AccessionGeneTitleLinks
X52228MUC1mucin 1, transmembraneSource|GeneCards
U82381
L08246MCL1myeloid cell leukemia sequence 1 (BCL2-related)Source|GeneCards
X54942CKS2CDC28 protein kinase regulatory subunit 2Source|GeneCards
AF010312LITAFlipopolysaccharide-induced TNF factorSource|GeneCards
AF029750TAPBPTAP binding protein (tapasin)Source|GeneCards
J03592SLC25A6solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6Source|GeneCards
L37043CSNK1Ecasein kinase 1, epsilonSource|GeneCards
X55733EIF4Beukaryotic translation initiation factor 4BSource|GeneCards
M26252PKM2pyruvate kinase, muscleSource|GeneCards
X65550MKI67antigen identified by monoclonal antibody Ki-67Source|GeneCards
X06409
V00599
AI540925
AF032456
X15334
D14874ADMadrenomedullinSource|GeneCards
Y07604NME4non-metastatic cells 4, protein expressed inSource|GeneCards
AI535946
U37408CTBP1C-terminal binding protein 1Source|GeneCards
AI074025
M30474
M59465TNFAIP3tumor necrosis factor, alpha-induced protein 3Source|GeneCards
M13929
AF055001HERPUD1homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1Source|GeneCards
X15187TRA1tumor rejection antigen (gp96) 1Source|GeneCards
AF006088ARPC5actin related protein 2/3 complex, subunit 5, 16kDaSource|GeneCards
U35340CRYBB1crystallin, beta B1Source|GeneCards
U77735PIM2pim-2 oncogeneSource|GeneCards
AF091890GPR161G protein-coupled receptor 161Source|GeneCards
W26056
AF075599
D14697FDPSfarnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase)Source|GeneCards
X02344
X62055PTPN6protein tyrosine phosphatase, non-receptor type 6Source|GeneCards
AB017644UBE2E3ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)Source|GeneCards
U85773PMM2phosphomannomutase 2Source|GeneCards
M58028UBE1ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing)Source|GeneCards
M33552LSP1lymphocyte-specific protein 1Source|GeneCards
U28386KPNA2karyopherin alpha 2 (RAG cohort 1, importin alpha 1)Source|GeneCards
D90144
L41498
AL031681
D11086IL2RGinterleukin 2 receptor, gamma (severe combined immunodeficiency)Source|GeneCards
Z93930
J03459LTA4Hleukotriene A4 hydrolaseSource|GeneCards
U28686RBM3RNA binding motif protein 3Source|GeneCards
J03473PARP1poly (ADP-ribose) polymerase family, member 1Source|GeneCards
Z19554VIMvimentinSource|GeneCards
M35011ITGB5integrin, beta 5Source|GeneCards
U72649BTG2BTG family, member 2Source|GeneCards
U94855EIF3S5eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDaSource|GeneCards
X69549ARHGDIBRho GDP dissociation inhibitor (GDI) betaSource|GeneCards
X58141
U15131ST5suppression of tumorigenicity 5Source|GeneCards

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