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Gene set: BCELL_ASTIER (C2:CLIN:0014)
Standard nameBCELL_ASTIER
Systematic nameC2:CLIN:0014
Brief descriptionGenes differentially induced in serum-starved precursor B leukemia cells subjected to beta1-integrin ligation with fibronectin versus those subjected to adhesion by poly-L-lysine.
CategoryC2: Curated
Sub-categoryCLIN: Clinical
Full description or Abstract

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The physical interactions between B cells and stromal cells from
the lymphoid tissue microenvironment are critical to the survival
of normal and malignant B cells. They are principally mediated
by integrins expressed on B cells and counterreceptors on stromal
cells. Specifically, alpha4beta1 integrin engagement rescues B
cells from physiological or drug-induced apoptosis. Therefore,
in order to understand the mechanisms by which integrins prevent
apoptosis in leukemia B cells, we compared the temporal gene expression
profiles induced by beta1-integrin ligation with fibronectin (Fn)
or adhesion by poly-L-Lysine in serum-starved precursor B leukemia
cells. Among the 38 selected differentially expressed genes, 6
genes involved in adhesion (VAV2, EPB41L1, CORO1A), proliferation
(FRAP1, CCT4), and intercellular communication (GJB3) were validated
by real-time quantitative polymerase chain reaction (RT-Q-PCR).
Gene expression modulation could also be validated at the protein
level for 5 other genes. We show that integrin stimulation up-regulated
FBI-1 expression but inhibited CD79a, Requiem, c-Fos, and caspase
7 induction when the cells underwent apoptosis. We further demonstrate
that Fn stimulation also inhibits caspase 3 activation but increases
XIAP and survivin expression. Moreover, integrin stimulation also
prevents caspase activation induced by doxorubicin. Therefore,
we identified genes modulated by adhesion of human precursor B
leukemia cells that regulate proliferation and apoptosis, highlighting
new pathways that might provide insights into future therapy aiming
at targeting apoptosis of leukemia cells. 
Publication URL12393420
External linksna
Keywords & MeSH headingsApoptosis/genetics,Cell Division/genetics,Time Factors,Oligonucleotide Array Sequence Analysis,Gene Expression Profiling,P.H.S.,Cell Adhesion/drug effects/genetics,B-Lymphocytes,Cell Communication/genetics,Tumor Cells,Humans,Leukemia,Signal Transduction/genetics,Cell Survival/genetics,pathology,genetics,Polylysine/pharmacology,Gene Expression Regulation,Pre-B-Cell,Cultured,Fibronectins/pharmacology
OrganismHuman
Contributed byKate Stafford
Source platformHG_U95Av2
Downloadgrp|xml|map
Genes

61 genes, 64 accessions (Toggle view)

AccessionGeneTitleLinks
35260_atMONDOAMlx interactorSource|GeneCards
31535_i_atNA
32556_atU2AF2U2 (RNU2) small nuclear RNA auxiliary factor 2Source|GeneCards
37598_atRASSF2Ras association (RalGDS/AF-6) domain family 2Source|GeneCards
33060_g_atNA
33464_atLOC283761Hypothetical protein LOC283761Source|GeneCards
35688_g_atMTCP1mature T-cell proliferation 1Source|GeneCards
1140_atITGAEintegrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)Source|GeneCards
37355_atSTARD3START domain containing 3Source|GeneCards
40963_atABCA4ATP-binding cassette, sub-family A (ABC1), member 4Source|GeneCards
38529_atACACBacetyl-Coenzyme A carboxylase betaSource|GeneCards
37885_atC2ORF27 /// MGC50273chromosome 2 open reading frame 27 /// MGC50273 proteinSource|GeneCards
40223_r_atKIAA0685KIAA0685Source|GeneCards
37036_atMDN1MDN1, midasin homolog (yeast)Source|GeneCards
33568_atCHRNB4Cholinergic receptor, nicotinic, beta polypeptide 4Source|GeneCards
32502_atGDPD5glycerophosphodiester phosphodiesterase domain containing 5Source|GeneCards
1704_atVAV2vav 2 oncogeneSource|GeneCards
36971_atRW1RW1 proteinSource|GeneCards
1789_atCOPS5COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)Source|GeneCards
35665_atPIK3C3phosphoinositide-3-kinase, class 3Source|GeneCards
33917_atEPB41L1erythrocyte membrane protein band 4.1-like 1Source|GeneCards
31389_atOSILoxidative stress induced likeSource|GeneCards
38617_atLIMK2LIM domain kinase 2Source|GeneCards
1532_g_atPFAAP5Phosphonoformate immuno-associated protein 5Source|GeneCards
36375_atODF1outer dense fiber of sperm tails 1Source|GeneCards
32646_atKIF21Bkinesin family member 21BSource|GeneCards
31816_atGAAglucosidase, alpha; acid (Pompe disease, glycogen storage disease type II)Source|GeneCards
40139_atFRAP1FK506 binding protein 12-rapamycin associated protein 1Source|GeneCards
38749_atLYPDC1LY6/PLAUR domain containing 1Source|GeneCards
1612_s_atJUNDjun D proto-oncogeneSource|GeneCards
32913_i_atC19ORF29chromosome 19 open reading frame 29Source|GeneCards
36958_atZYXzyxinSource|GeneCards
36577_atPLEKHC1pleckstrin homology domain containing, family C (with FERM domain) member 1Source|GeneCards
688_atPSMC1proteasome (prosome, macropain) 26S subunit, ATPase, 1Source|GeneCards
37194_atGATA2GATA binding protein 2Source|GeneCards
39688_atDPF2D4, zinc and double PHD fingers family 2Source|GeneCards
31414_atTTTY2testis-specific transcript, Y-linked 2Source|GeneCards
38281_atCASP7caspase 7, apoptosis-related cysteine proteaseSource|GeneCards
31775_atSFTPDsurfactant, pulmonary-associated protein DSource|GeneCards
1915_s_atFOSv-fos FBJ murine osteosarcoma viral oncogene homologSource|GeneCards
2084_s_atETV4ets variant gene 4 (E1A enhancer binding protein, E1AF)Source|GeneCards
41076_atGJB3gap junction protein, beta 3, 31kDa (connexin 31)Source|GeneCards
36398_atNA
39229_atSDCCAG1Serologically defined colon cancer antigen 1Source|GeneCards
32234_atTOR1Atorsin family 1, member A (torsin A)Source|GeneCards
33881_atACSL3Acyl-CoA synthetase long-chain family member 3Source|GeneCards
1692_s_atGFRA2GDNF family receptor alpha 2Source|GeneCards
218_atIKIK cytokine, down-regulator of HLA IISource|GeneCards
32594_atCCT4chaperonin containing TCP1, subunit 4 (delta)Source|GeneCards
38976_atCORO1Acoronin, actin binding protein, 1ASource|GeneCards
38767_atSPRY1sprouty homolog 1, antagonist of FGF signaling (Drosophila)Source|GeneCards
37033_s_atGPX1glutathione peroxidase 1Source|GeneCards
1846_atLGALS8lectin, galactoside-binding, soluble, 8 (galectin 8)Source|GeneCards
34703_f_atHHLA1HERV-H LTR-associating 1Source|GeneCards
39539_atZBTB7Zinc finger and BTB domain containing 7ASource|GeneCards
39657_atKRT4keratin 4Source|GeneCards
40584_atNUP88nucleoporin 88kDaSource|GeneCards
38018_g_atCD79ACD79A antigen (immunoglobulin-associated alpha)Source|GeneCards
35779_atVPS45Avacuolar protein sorting 45A (yeast)Source|GeneCards
40701_atUSP13ubiquitin specific protease 13 (isopeptidase T-3)Source|GeneCards
38728_atNUP205nucleoporin 205kDaSource|GeneCards
41134_atDLGAP4discs, large (Drosophila) homolog-associated protein 4Source|GeneCards
32963_s_atRRAGDRas-related GTP binding DSource|GeneCards
39784_atEIF2S1Eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDaSource|GeneCards


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