| Gene set: EMT_DOWN_JECHLINGER (C2:PERT:0008) | | Standard name | EMT_DOWN_JECHLINGER |
| Systematic name | C2:PERT:0008 |
| Brief description | Genes downregulated for epithelial plasticity in tumor progression |
| Category | C2: Curated |
| Sub-category | PERT: Experimental pertuberation |
| Full description or Abstract | Epithelial-to-mesenchymal transition (EMT), a switch of polarized
epithelial cells to a migratory, fibroblastoid phenotype, is increasingly
considered as an important event during malignant tumor progression
and metastasis. To identify molecular players involved in EMT
and metastasis, we performed expression profiling of a set of
combined in vitro/in vivo cellular models, based on clonal, fully
polarized mammary epithelial cells. Seven closely related cell
pairs were used, which were modified by defined oncogenes and/or
external factors and showed specific aspects of epithelial plasticity
relevant to cell migration, local invasion and metastasis. Since
mRNA levels do not necessarily reflect protein levels in cells,
we used an improved expression profiling method based on polysome-bound
RNA, suitable to analyse global gene expression on Affymetrix
chips. A substantial fraction of all regulated genes was found
to be exclusively controlled at the translational level. Furthermore,
profiling of the above multiple cell pairs allowed one to identify
small numbers of genes by cluster analysis, specifically correlating
gene expression with EMT, metastasis, scattering and/or oncogene
function. A small set of genes specifically regulated during EMT
was identified, including key regulators and signaling pathways
involved in cell proliferation, epithelial polarity, survival
and trans-differentiation to mesenchymal-like cells with invasive
behavior. |
| Publication URL | 14562044 |
| External links | na |
| Keywords & MeSH headings | Neoplastic,Epithelial Cells,Animal/cytology/pathology,Gene Expression Profiling,Cell Transformation,Messenger/genetics,Human,Support,ras,Translation,Genes,genetics,Cell Differentiation,Mice,Genetic,Cluster Analysis,Cell Line,Mesoderm,RNA,Breast Neoplasms/genetics/pathology,Mammary Glands,Polyribosomes/genetics,Female,Transcription,pathology,Neoplasm Invasiveness,Gene Expression Regulation,physiology |
| Organism | Human |
| Contributed by | Broad Institute |
| Source platform | Mu11KsubA |
| Download | grp|xml|map |
| Genes | 40 genes, 40 accessions (Toggle view) | Accession | Gene | Title | Links |
|---|
| FBP2 | | HMMR | | STAT5A | | FLNA | | BCL6 | | ATF3 | | MYH9 | | FOSB | | TIMP3 | | RARA | | IRF6 | | AMD1 | | F3 | | PKP1 | | EGR1 | | TGM2 | | ATP1A1 | | DUSP1 | | ITPR1 | | SGK | | CTGF | | KITLG | | SERPINB5 | | ACTN4 | | NUMB | | JUP | | PADI2 | | VAMP8 | | KLF2 | | TACSTD1 | | TGFB3 | | ARHGEF1 | | NNT | | THBS1 | | CDH1 | | TIAM1 | | GRB7 | | ITGB5 | | CTSH | | EGR2 |
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