| Gene set: IFNG_5ENDOTHELIAL_UP (C2:PERT:0131) | | Standard name | IFNG_5ENDOTHELIAL_UP |
| Systematic name | C2:PERT:0131 |
| Brief description | Genes up-regulated by interferon-gamma in colon,derm,iliac,aortic,lung endothelial cells |
| Category | C2: Curated |
| Sub-category | PERT: Experimental pertuberation |
| Full description or Abstract | Toggle abstract view To investigate the potential molecular mediators of tissue-specific
recruitment, we explored the influence of different cytokine challenges
on gene expression regulation in five primary endothelial cells
(ECs), representing two different phenotypes: iliac artery and
aortic (macrovascular); lung, colon and dermal (microvascular).
We challenged ECs with cytokines that elicit different patterns
of inflammatory and immune responses in immune cells: tumor necrosis
factor (TNF-alpha), interferon-gamma (IFN-gamma) or interleukin-4
(IL-4), and used microarrays containing approximately 40,000 unique
cDNAs, to assess changes in differential gene expression relative
to untreated cells. Five hundred and sixty three sequences changed
by at least 2.5 fold in one or more of the 15 possible EC /cytokine
combinations. The list included highly regulated adhesion molecules,
chemokines, cytokines, metalloproteases, and IFN-gamma-induced
genes. Overall, IFN-gamma caused the largest number of gene expression
changes and its profile was least correlated with IL-4. In addition
to clusters that were predominantly EC/cytokine specific, we also
observed several clusters that were regulated by more than one
cytokine across several ECs. Furthermore, we identified genes
that were reciprocally expressed in response to different cytokines
that could serve as markers of inflammatory and immune expression.
These results confirm the importance of microenvironment in primary
ECs that could have important applications in developing targeted
therapies for vascular diseases. |
| Publication URL | 15749026 |
| External links | na |
| Keywords & MeSH headings | Vascular,Endothelial Cells/drug effects/metabolism,Gene Expression Profiling,Oligonucleotide Array Sequence Analysis,pharmacology,Endothelium,Interferon Type II/pharmacology,immunology,Humans,Interleukin-4/pharmacology,Polymerase Chain Reaction,Gene Expression Regulation,cytology,Cytokines,Inflammation Mediators/pharmacology |
| Organism | Human |
| Contributed by | Yujin Hoshida |
| Source platform | Gene_Symbol |
| Download | grp|xml|map |
| Genes | 63 genes, 100 accessions (Toggle view) |