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Gene set: IL5_DOWN_BYSTROM (C2:PERT:0061)
Standard nameIL5_DOWN_BYSTROM
Systematic nameC2:PERT:0061
Brief descriptionGenes downregulated in mouse bone marrow in response to interleukin-5.
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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Interleukin-5 (IL-5) is a hematopoietic differentiation factor
that promotes the development of mature eosinophils from progenitors
in bone marrow. We present a multifactorial microarray study documenting
the transcriptional events in bone marrow of wild-type and IL-5-deficient
mice at baseline and in response to infection with Schistosoma
mansoni. The microarray data were analyzed by a 4-way subtractive
algorithm that eliminated confounding non-IL-5-related sequelae
of schistosome infection as well as alterations in gene expression
among uninfected mice. Among the most prominent findings, we observed
7- to 40-fold increased expression of transcripts encoding the
classic eosinophil granule proteins (eosinophil peroxidase, major
basic protein, the ribonucleases) together with arachidonate-15-lipoxygenase
and protease inhibitor plasminogen activator inhibitor 2 (PAI-2),
in the IL-5-producing, infected wild-type mice only. This was
accompanied by increased transcription of genes involved in secretory
protein biosynthesis and granule-vesicle formation. Interestingly,
we did not detect increased expression of genes encoding eosinophil-related
chemokine receptors (CCR1, CCR3) or members of the GATA or CCAAT/enhancer
binding protein (C/EBP) transcription factor families. These data
suggest that the IL-5-responsive progenitors in the mouse bone
marrow are already significantly committed to the eosinophil lineage
and that IL-5 promotes differentiation of these committed progenitors
into cells with recognizable and characteristic cytoplasmic granules
and granule proteins. 
Publication URL14525773
External linksna
Keywords & MeSH headingsKnockout,Oligonucleotide Array Sequence Analysis,Gene Expression Profiling,P.H.S.,Blood Proteins/genetics,Complementary/genetics,immunology,Hematopoietic Stem Cells,genetics,Genetic,Mice,Hematopoiesis,deficiency,Schistosomiasis mansoni/genetics/immunology,metabolism,Eosinophilia/etiology,DNA,Base Sequence,Transcription,Inbred C57BL,Ribonucleases/genetics,Eosinophil Granule Proteins,Reverse Transcriptase Polymerase Chain Reaction,Interleukin-5,cytology
OrganismMouse
Contributed byKate Stafford
Source platformSeq_Accession
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Genes

73 genes, 73 accessions (Toggle view)

AccessionGeneTitleLinks
SF3B1SF3B1Splicing factor 3b, subunit 1, 155kDaSource|GeneCards
AF043939
A1325802
WRNWRNWerner syndromeSource|GeneCards
L28060
FOXM1FOXM1forkhead box M1Source|GeneCards
TMPOTMPOthymopoietinSource|GeneCards
LYSTLYSTlysosomal trafficking regulatorSource|GeneCards
RARARARAretinoic acid receptor, alphaSource|GeneCards
PRKREIF2AK2eukaryotic translation initiation factor 2-alpha kinase 2Source|GeneCards
KPNA3KPNA3karyopherin alpha 3 (importin alpha 4)Source|GeneCards
PTPRCPTPRCprotein tyrosine phosphatase, receptor type, CSource|GeneCards
IL6STIL6STinterleukin 6 signal transducer (gp130, oncostatin M receptor)Source|GeneCards
INCENPINCENPinner centromere protein antigens 135/155kDaSource|GeneCards
SLASLASrc-like-adaptorSource|GeneCards
PSTPIP2PSTPIP2proline-serine-threonine phosphatase interacting protein 2Source|GeneCards
SORL1SORL1sortilin-related receptor, L(DLR class) A repeats-containingSource|GeneCards
TRAM1TRAM1Translocation associated membrane protein 1Source|GeneCards
PIK3R1PIK3R1phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)Source|GeneCards
CASP7CASP7caspase 7, apoptosis-related cysteine proteaseSource|GeneCards
ZFA
PIM1PIM1pim-1 oncogeneSource|GeneCards
BCL2BCL2B-cell CLL/lymphoma 2Source|GeneCards
STAT5BSTAT5Bsignal transducer and activator of transcription 5BSource|GeneCards
HCFC1HCFC1host cell factor C1 (VP16-accessory protein)Source|GeneCards
L43568
BAZ1BBAZ1Bbromodomain adjacent to zinc finger domain, 1BSource|GeneCards
SYKSYKspleen tyrosine kinaseSource|GeneCards
CLK4CLK4CDC-like kinase 4Source|GeneCards
ERCC2ERCC2excision repair cross-complementing rodent repair deficiency, complementation group 2 (xeroderma pigmentosum D)Source|GeneCards
HIPK1HIPK1homeodomain interacting protein kinase 1Source|GeneCards
DDB1DDB1damage-specific DNA binding protein 1, 127kDaSource|GeneCards
ADARADARadenosine deaminase, RNA-specificSource|GeneCards
SFRS1SFRS1splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor)Source|GeneCards
NR3C1NR3C1nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)Source|GeneCards
CD44CD44CD44 antigen (homing function and Indian blood group system)Source|GeneCards
MTMR1MTMR1myotubularin related protein 1Source|GeneCards
PITPNBPITPNBPhosphatidylinositol transfer protein, betaSource|GeneCards
AP1G1AP1G1adaptor-related protein complex 1, gamma 1 subunitSource|GeneCards
PAPOLAPAPOLApoly(A) polymerase alphaSource|GeneCards
RNF2RNF2ring finger protein 2Source|GeneCards
SSR3SSR3signal sequence receptor, gamma (translocon-associated protein gamma)Source|GeneCards
LY78LY64lymphocyte antigen 64 homolog, radioprotective 105kDa (mouse)Source|GeneCards
ADAM10ADAM10a disintegrin and metalloproteinase domain 10Source|GeneCards
RNF13RNF13ring finger protein 13Source|GeneCards
KLRK1KLRK1killer cell lectin-like receptor subfamily K, member 1Source|GeneCards
SNX2SNX2sorting nexin 2Source|GeneCards
IKBKBIKBKBinhibitor of kappa light polypeptide gene enhancer in B-cells, kinase betaSource|GeneCards
PTPRJPTPRJprotein tyrosine phosphatase, receptor type, JSource|GeneCards
PIK3CDPIK3CDphosphoinositide-3-kinase, catalytic, delta polypeptideSource|GeneCards
HIVEP2HIVEP2human immunodeficiency virus type I enhancer binding protein 2Source|GeneCards
MATR3MATR3matrin 3Source|GeneCards
ZFPN1A1ZFPN1A1zinc finger protein, subfamily 1A, 1 (Ikaros)Source|GeneCards
CNOT7CNOT7CCR4-NOT transcription complex, subunit 7Source|GeneCards
D10ERTD516E
L33954
TGOLN1TGOLN1trans-golgi network protein 1Source|GeneCards
ITGAMITGAMintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide)Source|GeneCards
RAB10RAB10RAB10, member RAS oncogene familySource|GeneCards
PLD1PLD1phospholipase D1, phophatidylcholine-specificSource|GeneCards
CYBBCYBBcytochrome b-245, beta polypeptide (chronic granulomatous disease)Source|GeneCards
MADAMPD1adenosine monophosphate deaminase 1 (isoform M)Source|GeneCards
CEACAM1CEACAM1carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)Source|GeneCards
NCF1NCF1neutrophil cytosolic factor 1 (47kDa, chronic granulomatous disease, autosomal 1)Source|GeneCards
MAPK9MAPK9mitogen-activated protein kinase 9Source|GeneCards
ZFP422ZNF22zinc finger protein 22 (KOX 15)Source|GeneCards
ARNTARNTaryl hydrocarbon receptor nuclear translocatorSource|GeneCards
IGH-VJ558
EIF4G1EIF4G1eukaryotic translation initiation factor 4 gamma, 1Source|GeneCards
MAPKAPK2MAPKAPK2mitogen-activated protein kinase-activated protein kinase 2Source|GeneCards
SLFN3FLJ10260hypothetical protein FLJ10260Source|GeneCards
PKNOX1PKNOX1PBX/knotted 1 homeobox 1Source|GeneCards
ITGALITGALintegrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)Source|GeneCards


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