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Gene set: PASSERINI_INFLAMMATION (C2:PERT:0081)
Standard namePASSERINI_INFLAMMATION
Systematic nameC2:PERT:0081
Brief descriptionGenes associated with cellular adhesion that are differentially expressed in endothelial cells of pig aortas from regions of disturbed flow (inner aortic arch) versus regions of undisturbed laminar flow (descending thoracic aorta).
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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In the arterial circulation, regions of disturbed flow (DF), which
are characterized by flow separation and transient vortices, are
susceptible to atherogenesis, whereas regions of undisturbed laminar
flow (UF) appear protected. Coordinated regulation of gene expression
by endothelial cells (EC) may result in differing regional phenotypes
that either favor or inhibit atherogenesis. Linearly amplified
RNA from freshly isolated EC of DF (inner aortic arch) and UF
(descending thoracic aorta) regions of normal adult pigs was used
to profile differential gene expression reflecting the steady
state in vivo. By using human cDNA arrays, approximately 2,000
putatively differentially expressed genes were identified through
false-discovery-rate statistical methods. A sampling of these
genes was validated by quantitative real-time PCR and/or immunostaining
en face. Biological pathway analysis revealed that in DF there
was up-regulation of several broad-acting inflammatory cytokines
and receptors, in addition to elements of the NF-kappaB system,
which is consistent with a proinflammatory phenotype. However,
the NF-kappaB complex was predominantly cytoplasmic (inactive)
in both regions, and no significant differences were observed
in the expression of key adhesion molecules for inflammatory cells
associated with early atherogenesis. Furthermore, there was no
histological evidence of inflammation. Protective profiles were
observed in DF regions, notably an enhanced antioxidative gene
expression. This study provides a public database of regional
EC gene expression in a normal animal, implicates hemodynamics
as a contributory mechanism to athero-susceptibility, and reveals
the coexistence of pro- and antiatherosclerotic transcript profiles
in susceptible regions. The introduction of additional risk factors
may shift this balance to favor lesion development. 
Publication URL14983035
External linksna
Keywords & MeSH headingsComputational Biology,Vascular,Apoptosis/genetics,Male,Non-P.H.S.,Gene Expression Profiling,P.H.S.,Enzymes/genetics,Transcription,Polymerase Chain Reaction/methods,Endothelium,Swine,Regional Blood Flow,Proteins/genetics,genetics,Genetic,Gene Expression Regulation,physiology,Oxidation-Reduction,Aorta,Reproducibility of Results
OrganismPig
Contributed byKate Stafford
Source platformSeq_Accession
Downloadgrp|xml|map
Genes

22 genes, 26 accessions (Toggle view)

AccessionGeneTitleLinks
X04011CYBBcytochrome b-245, beta polypeptide (chronic granulomatous disease)Source|GeneCards
AF153419IKBKAPinhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated proteinSource|GeneCards
L19593IL8RBinterleukin 8 receptor, betaSource|GeneCards
D10202PTAFRplatelet-activating factor receptorSource|GeneCards
BC010954CXCL10chemokine (C-X-C motif) ligand 10Source|GeneCards
D28235PTGS2prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)Source|GeneCards
M31165TNFAIP6tumor necrosis factor, alpha-induced protein 6Source|GeneCards
BC004983NFKBIAnuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alphaSource|GeneCards
X02851IL1Ainterleukin 1, alphaSource|GeneCards
BC004490FOSv-fos FBJ murine osteosarcoma viral oncogene homologSource|GeneCards
M99412
U76764CD97CD97 antigenSource|GeneCards
BC010623NFE2L1nuclear factor (erythroid-derived 2)-like 1Source|GeneCards
AF282693GPR84G protein-coupled receptor 84Source|GeneCards
X13334CD14CD14 antigenSource|GeneCards
AJ293573ZA20D1zinc finger, A20 domain containing 1Source|GeneCards
L35545PROCRprotein C receptor, endothelial (EPCR)Source|GeneCards
M58603NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)Source|GeneCards
R81994
S76638NFKB2nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)Source|GeneCards
M37719
H12612
M27492IL1R1interleukin 1 receptor, type ISource|GeneCards
X76981ADORA3adenosine A3 receptorSource|GeneCards
AB036432AGERadvanced glycosylation end product-specific receptorSource|GeneCards
AF005058CXCR4chemokine (C-X-C motif) receptor 4Source|GeneCards


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