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Gene set: PASSERINI_PROLIFERATION (C2:PERT:0083)
Standard namePASSERINI_PROLIFERATION
Systematic nameC2:PERT:0083
Brief descriptionGenes associated with cellular adhesion that are differentially expressed in endothelial cells of pig aortas from regions of disturbed flow (inner aortic arch) versus regions of undisturbed laminar flow (descending thoracic aorta).
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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In the arterial circulation, regions of disturbed flow (DF), which
are characterized by flow separation and transient vortices, are
susceptible to atherogenesis, whereas regions of undisturbed laminar
flow (UF) appear protected. Coordinated regulation of gene expression
by endothelial cells (EC) may result in differing regional phenotypes
that either favor or inhibit atherogenesis. Linearly amplified
RNA from freshly isolated EC of DF (inner aortic arch) and UF
(descending thoracic aorta) regions of normal adult pigs was used
to profile differential gene expression reflecting the steady
state in vivo. By using human cDNA arrays, approximately 2,000
putatively differentially expressed genes were identified through
false-discovery-rate statistical methods. A sampling of these
genes was validated by quantitative real-time PCR and/or immunostaining
en face. Biological pathway analysis revealed that in DF there
was up-regulation of several broad-acting inflammatory cytokines
and receptors, in addition to elements of the NF-kappaB system,
which is consistent with a proinflammatory phenotype. However,
the NF-kappaB complex was predominantly cytoplasmic (inactive)
in both regions, and no significant differences were observed
in the expression of key adhesion molecules for inflammatory cells
associated with early atherogenesis. Furthermore, there was no
histological evidence of inflammation. Protective profiles were
observed in DF regions, notably an enhanced antioxidative gene
expression. This study provides a public database of regional
EC gene expression in a normal animal, implicates hemodynamics
as a contributory mechanism to athero-susceptibility, and reveals
the coexistence of pro- and antiatherosclerotic transcript profiles
in susceptible regions. The introduction of additional risk factors
may shift this balance to favor lesion development. 
Publication URL14983035
External linksna
Keywords & MeSH headingsComputational Biology,Vascular,Apoptosis/genetics,Male,Non-P.H.S.,Gene Expression Profiling,P.H.S.,Enzymes/genetics,Transcription,Polymerase Chain Reaction/methods,Endothelium,Swine,Regional Blood Flow,Proteins/genetics,genetics,Genetic,Gene Expression Regulation,physiology,Oxidation-Reduction,Aorta,Reproducibility of Results
OrganismPig
Contributed byKate Stafford
Source platformSeq_Accession
Downloadgrp|xml|map
Genes

60 genes, 67 accessions (Toggle view)

AccessionGeneTitleLinks
S75725IGFBP7insulin-like growth factor binding protein 7Source|GeneCards
AF231124SPOCKsparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican)Source|GeneCards
X54936PGFplacental growth factor, vascular endothelial growth factor-related proteinSource|GeneCards
L19593IL8RBinterleukin 8 receptor, betaSource|GeneCards
BC010954CXCL10chemokine (C-X-C motif) ligand 10Source|GeneCards
X05199PLGplasminogenSource|GeneCards
AK001630ETS1v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)Source|GeneCards
AF060153ADAMTS8a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8Source|GeneCards
D38305TOB1transducer of ERBB2, 1Source|GeneCards
M60278HBEGFheparin-binding EGF-like growth factorSource|GeneCards
L15409VHLvon Hippel-Lindau syndromeSource|GeneCards
AF049140UBE2V2ubiquitin-conjugating enzyme E2 variant 2Source|GeneCards
X02851IL1Ainterleukin 1, alphaSource|GeneCards
X04430IL6interleukin 6 (interferon, beta 2)Source|GeneCards
M15796PCNAproliferating cell nuclear antigenSource|GeneCards
Y00083TGFB2transforming growth factor, beta 2Source|GeneCards
D90224TNFSF4tumor necrosis factor (ligand) superfamily, member 4 (tax-transcriptionally activated glycoprotein 1, 34kDa)Source|GeneCards
U24166MAPRE1microtubule-associated protein, RP/EB family, member 1Source|GeneCards
U87269E4F1E4F transcription factor 1Source|GeneCards
U03057FSCN1fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)Source|GeneCards
X77753TACSTD2tumor-associated calcium signal transducer 2Source|GeneCards
D11428
U22398CDKN1Ccyclin-dependent kinase inhibitor 1C (p57, Kip2)Source|GeneCards
AF369657NF2neurofibromin 2 (bilateral acoustic neuroma)Source|GeneCards
Y18046FGFR1OPFGFR1 oncogene partnerSource|GeneCards
M57627IL10interleukin 10Source|GeneCards
S56805EDN1endothelin 1Source|GeneCards
AF062536CUL1cullin 1Source|GeneCards
U02020PBEF1pre-B-cell colony enhancing factor 1Source|GeneCards
U03106CDKN1Acyclin-dependent kinase inhibitor 1A (p21, Cip1)Source|GeneCards
M13994BCL2B-cell CLL/lymphoma 2Source|GeneCards
X04571EGFepidermal growth factor (beta-urogastrone)Source|GeneCards
BC001971CDKN1Bcyclin-dependent kinase inhibitor 1B (p27, Kip1)Source|GeneCards
BC005155ARHGEF1Rho guanine nucleotide exchange factor (GEF) 1Source|GeneCards
U77085EMP1epithelial membrane protein 1Source|GeneCards
AF055479TUSC2tumor suppressor candidate 2Source|GeneCards
M34065CDC25Ccell division cycle 25CSource|GeneCards
U01134FLT1fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)Source|GeneCards
AF083033PRKRAprotein kinase, interferon-inducible double stranded RNA dependent activatorSource|GeneCards
AF065389TM4SF9transmembrane 4 superfamily member 9Source|GeneCards
BC001293HOXC10homeo box C10Source|GeneCards
X94216VEGFCvascular endothelial growth factor CSource|GeneCards
U78305PPM1Dprotein phosphatase 1D magnesium-dependent, delta isoformSource|GeneCards
D29956USP8ubiquitin specific protease 8Source|GeneCards
X52479PRKCAprotein kinase C, alphaSource|GeneCards
M21574PDGFRAplatelet-derived growth factor receptor, alpha polypeptideSource|GeneCards
M27968
M99412
AF019047TNFSF11tumor necrosis factor (ligand) superfamily, member 11Source|GeneCards
AF041248CDKN2Ccyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)Source|GeneCards
BC000866TIMP1tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)Source|GeneCards
BC008308CUL4Acullin 4ASource|GeneCards
U02687FLT3fms-related tyrosine kinase 3Source|GeneCards
AB013924LAMP3lysosomal-associated membrane protein 3Source|GeneCards
AF083811MAD1L1MAD1 mitotic arrest deficient-like 1 (yeast)Source|GeneCards
X70340TGFAtransforming growth factor, alphaSource|GeneCards
D87462
BC007280NUDCnuclear distribution gene C homolog (A. nidulans)Source|GeneCards
X51602FLT1fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)Source|GeneCards
AK025306
M31774TSHRthyroid stimulating hormone receptorSource|GeneCards
U03187IL12RB1interleukin 12 receptor, beta 1Source|GeneCards
L27211CDKN2Acyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)Source|GeneCards
L49207PTK2BPTK2B protein tyrosine kinase 2 betaSource|GeneCards
BC001422PGFplacental growth factor, vascular endothelial growth factor-related proteinSource|GeneCards
AF105036KLF4Kruppel-like factor 4 (gut)Source|GeneCards
AF013970CBFA2T2core-binding factor, runt domain, alpha subunit 2; translocated to, 2Source|GeneCards


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