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Gene set: PASSERINI_TRANSCRIPTION (C2:PERT:0085)
Standard namePASSERINI_TRANSCRIPTION
Systematic nameC2:PERT:0085
Brief descriptionGenes associated with cellular adhesion that are differentially expressed in endothelial cells of pig aortas from regions of disturbed flow (inner aortic arch) versus regions of undisturbed laminar flow (descending thoracic aorta).
CategoryC2: Curated
Sub-categoryPERT: Experimental pertuberation
Full description or Abstract

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In the arterial circulation, regions of disturbed flow (DF), which
are characterized by flow separation and transient vortices, are
susceptible to atherogenesis, whereas regions of undisturbed laminar
flow (UF) appear protected. Coordinated regulation of gene expression
by endothelial cells (EC) may result in differing regional phenotypes
that either favor or inhibit atherogenesis. Linearly amplified
RNA from freshly isolated EC of DF (inner aortic arch) and UF
(descending thoracic aorta) regions of normal adult pigs was used
to profile differential gene expression reflecting the steady
state in vivo. By using human cDNA arrays, approximately 2,000
putatively differentially expressed genes were identified through
false-discovery-rate statistical methods. A sampling of these
genes was validated by quantitative real-time PCR and/or immunostaining
en face. Biological pathway analysis revealed that in DF there
was up-regulation of several broad-acting inflammatory cytokines
and receptors, in addition to elements of the NF-kappaB system,
which is consistent with a proinflammatory phenotype. However,
the NF-kappaB complex was predominantly cytoplasmic (inactive)
in both regions, and no significant differences were observed
in the expression of key adhesion molecules for inflammatory cells
associated with early atherogenesis. Furthermore, there was no
histological evidence of inflammation. Protective profiles were
observed in DF regions, notably an enhanced antioxidative gene
expression. This study provides a public database of regional
EC gene expression in a normal animal, implicates hemodynamics
as a contributory mechanism to athero-susceptibility, and reveals
the coexistence of pro- and antiatherosclerotic transcript profiles
in susceptible regions. The introduction of additional risk factors
may shift this balance to favor lesion development. 
Publication URL14983035
External linksna
Keywords & MeSH headingsComputational Biology,Vascular,Apoptosis/genetics,Male,Non-P.H.S.,Gene Expression Profiling,P.H.S.,Enzymes/genetics,Transcription,Polymerase Chain Reaction/methods,Endothelium,Swine,Regional Blood Flow,Proteins/genetics,genetics,Genetic,Gene Expression Regulation,physiology,Oxidation-Reduction,Aorta,Reproducibility of Results
OrganismPig
Contributed byKate Stafford
Source platformSeq_Accession
Downloadgrp|xml|map
Genes

67 genes, 82 accessions (Toggle view)

AccessionGeneTitleLinks
U35052
AF142482TEAD3TEA domain family member 3Source|GeneCards
R82817
AF032885FOXO1Aforkhead box O1A (rhabdomyosarcoma)Source|GeneCards
AF307851TP53tumor protein p53 (Li-Fraumeni syndrome)Source|GeneCards
AB006909MITFmicrophthalmia-associated transcription factorSource|GeneCards
AK001630ETS1v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)Source|GeneCards
U80017
AJ132592ZNF281zinc finger protein 281Source|GeneCards
L15409VHLvon Hippel-Lindau syndromeSource|GeneCards
H13139
BC004490FOSv-fos FBJ murine osteosarcoma viral oncogene homologSource|GeneCards
X04707THRBthyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)Source|GeneCards
AF039081CREBL2cAMP responsive element binding protein-like 2Source|GeneCards
D31716KLF9Kruppel-like factor 9Source|GeneCards
S57212MEF2CMADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C)Source|GeneCards
U60477
U78521AIParyl hydrocarbon receptor interacting proteinSource|GeneCards
U22680
U87269E4F1E4F transcription factor 1Source|GeneCards
Y08976FEVFEV (ETS oncogene family)Source|GeneCards
AF164104SOX8SRY (sex determining region Y)-box 8Source|GeneCards
M31627XBP1X-box binding protein 1Source|GeneCards
L12699EN1engrailed homolog 1Source|GeneCards
M58603NFKB1nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)Source|GeneCards
AB013897HKR1GLI-Kruppel family member HKR1Source|GeneCards
S76638NFKB2nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)Source|GeneCards
U73328DLX4distal-less homeobox 4Source|GeneCards
M59079NFYAnuclear transcription factor Y, alphaSource|GeneCards
AF084530DMTF1cyclin D binding myb-like transcription factor 1Source|GeneCards
M31899ERCC3excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing)Source|GeneCards
U05040FUBP1far upstream element (FUSE) binding protein 1Source|GeneCards
U75308TAF4TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDaSource|GeneCards
AJ279081C21ORF66chromosome 21 open reading frame 66Source|GeneCards
BC002712MYCNv-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)Source|GeneCards
U02619GTF3C1general transcription factor IIIC, polypeptide 1, alpha 220kDaSource|GeneCards
M92299HOXB5homeo box B5Source|GeneCards
X59842
U68019SMAD3SMAD, mothers against DPP homolog 3 (Drosophila)Source|GeneCards
U10990
AB025432DSIPIdelta sleep inducing peptide, immunoreactorSource|GeneCards
AJ007989TBX6T-box 6Source|GeneCards
U85707MEIS1Meis1, myeloid ecotropic viral integration site 1 homolog (mouse)Source|GeneCards
L08895MEF2CMADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C)Source|GeneCards
L27586NR2C2nuclear receptor subfamily 2, group C, member 2Source|GeneCards
AF071538SPDEFSAM pointed domain containing ets transcription factorSource|GeneCards
M87503ISGF3Ginterferon-stimulated transcription factor 3, gamma 48kDaSource|GeneCards
AF039196HRhairless homolog (mouse)Source|GeneCards
X95701GATA6GATA binding protein 6Source|GeneCards
X59373HOXD10homeo box D10Source|GeneCards
M97936STAT1signal transducer and activator of transcription 1, 91kDaSource|GeneCards
L46720ENPP2ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin)Source|GeneCards
X56465ZNF6zinc finger protein 6 (CMPX1)Source|GeneCards
BC004983NFKBIAnuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alphaSource|GeneCards
BC001293HOXC10homeo box C10Source|GeneCards
Y10871TWIST1twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)Source|GeneCards
X74142FOXG1Bforkhead box G1BSource|GeneCards
BC010623NFE2L1nuclear factor (erythroid-derived 2)-like 1Source|GeneCards
U17163ETV1ets variant gene 1Source|GeneCards
H04341
BC001167RXRBretinoid X receptor, betaSource|GeneCards
AF087940HAND2heart and neural crest derivatives expressed 2Source|GeneCards
AF033342
L13773AFF1AF4/FMR2 family, member 1Source|GeneCards
X17651MYOGmyogenin (myogenic factor 4)Source|GeneCards
U09850ZNF143zinc finger protein 143 (clone pHZ-1)Source|GeneCards
D14041
U25435CTCFCCCTC-binding factor (zinc finger protein)Source|GeneCards
R81714
AJ001388ZNF238zinc finger protein 238Source|GeneCards
AF287272KLF5Kruppel-like factor 5 (intestinal)Source|GeneCards
U44754SNAPC1small nuclear RNA activating complex, polypeptide 1, 43kDaSource|GeneCards
AF230388TRIM29tripartite motif-containing 29Source|GeneCards
BC008727RARAretinoic acid receptor, alphaSource|GeneCards
L29277STAT3signal transducer and activator of transcription 3 (acute-phase response factor)Source|GeneCards
X87613BRD8bromodomain containing 8Source|GeneCards
AF178948MEIS2Meis1, myeloid ecotropic viral integration site 1 homolog 2 (mouse)Source|GeneCards
AJ245587
J04111JUNv-jun sarcoma virus 17 oncogene homolog (avian)Source|GeneCards
X99268TWIST1twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)Source|GeneCards
M69238ARNTaryl hydrocarbon receptor nuclear translocatorSource|GeneCards
AF105036KLF4Kruppel-like factor 4 (gut)Source|GeneCards


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