XX
XX
XX
FA C/EBPbeta
XX
SY AGP/EBP; ANF-2;
C/EBPbeta; CRP2; H-APF-2; IL-6DBP; LAP; LAP1; NF-IL6; NF-M;
SY C/EBP-beta.
XX
OS human, Homo sapiens
OC eukaryota;
animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia;
OC eutheria; primates
XX
XX
CL C0008; bZIP; 1.1.3.0.2.1.
XX
SZ 345 AA; 36.1 kDa (cDNA)
XX
XX
SC SwissProt
#P17676
XX
FT 9 19 proline-rich region (7/11).
FT
46 59 proline-rich region (7/14).
FT 81 108 proline-rich region
(9/28).
FT 154 181 proline-rich region (13/28).
FT 218
237 serine-rich region (10/14).
FT 235 235 Thr-phosphorylation
by MAP kinases [5].
FT 260 295 basic region.
FT 266 272
complex stabilizing subdomain [2].
FT 266 345 tryptic core
domain within DNA complex [2].
FT 269 333 PF00170; bZIP.
FT 269 333 SM00338; BRLZ.
FT 299 334 leucine zipper (TL5).
FT 303 345 tryptic core domain.
XX
SF very relaxed DNA-binding
specificity, may even bind to AP-1 sites and may
SF heterodimerize
with either c-Fos or c-Jun [3];
SF the leucine zipper motif
may also interact with the Rel-domain of
SF NF-kappaB1 (p50)
thus causing synergistic effects of both factors [7] [6];
SF interaction with hypophosphorylated Rb protein enhances DNA-binding
and
SF trans-activation [1];
XX
CP widespread; most
abundant in spleen; liver, kidney after stimulation [9];
CP
myeloid cells
XX
FF transcriptional activator in vitro
and in vivo [9];
FF involved in acute phase reactions, inflammation
and haematopoiesis;
FF induced by IL-1, IL-6, LPS [9];
FF master regulator of the acute-phase response [9];
FF regulates
constitutive as well as IL-1-induced transcription of IL-6 [9];
FF at the IL-6 promoter, it acts antagonistically to the p53 tumor
suppressor
FF [4];
FF protein is abundant in liver due
to posttranscriptional regulation [9];
FF phosphorylation
by p21(ras)-dependent MAP kinases enhances transcriptional
FF activation [5];
FF trans-activation is repressed by association
with c-Fos or c-Jun [3];
XX
IN T01303; ATF4; human, Homo
sapiens.
IN T00123; c-Fos; human, Homo sapiens.
IN T00133;
c-Jun; human, Homo sapiens.
IN T00105; C/EBPalpha; human,
Homo sapiens.
IN T00108; C/EBPalpha; rat, Rattus norvegicus.
IN T00109; C/EBPdelta; rat, Rattus norvegicus.
IN T00583;
C/EBPdelta; human, Homo sapiens.
IN T01114; C/EBPdelta; mouse,
Mus musculus.
IN T00127; CHOP-10; mouse, Mus musculus.
IN T00593; NF-kappaB1; human, Homo sapiens.
IN T00722; pRb;
human, Homo sapiens.
IN T00594; RelA; human, Homo sapiens.
XX
XX
XX
XX
RN [1]; RE0006633.
RX MEDLINE;
96133958.
RA Chen P.-L., Riley D. J., Chen-Kiang S., Lee W.-H.
RT Retinoblastoma protein directly interacts with and activates
the
RT transcription factor NF-IL6
RL Proc. Natl. Acad.
Sci. USA 93:465-469 (1996).
RN [2]; RE0001110.
RX MEDLINE;
94193722.
RA Brasier A. R., Kumar A.
RT Identification
of a novel determinant for basic domain-leucine zipper DNA
RT binding activity in the acute-phase inducible nuclear factor-interleukin-6
RT transcription factor
RL J. Biol. Chem. 269:10341-10351
(1994).
RN [3]; RE0004730.
RX MEDLINE; 94088523.
RA
Hsu W., Kerppola T. K., Chen P.-L., Curran T., Chen-Kiang S.
RT Fos and Jun repress transcription activation by NF-IL6 through
association
RT at the basic zipper region
RL Mol. Cell.
Biol. 14:268-276 (1994).
RN [4]; RE0003029.
RX MEDLINE;
93315489.
RA Margulies L., Sehgal P. B.
RT Modulation
of the human interleukin-6 promoter (IL-6) and transcription
RT factor C/EBPbeta (NF-IL-6) activity by p53 species
RL J.
Biol. Chem. 268:15096-15100 (1993).
RN [5]; RE0003030.
RX MEDLINE; 93211931.
RA Nakajima T., Kinoshita S., Sasagawa
T., Sasaki K., Naruto M., Kishimoto T.,
RA Akira S.
RT
Phosphorylation at threonine-235 by ras-dependent mitogen-activated
protein
RT kinase cascade is essential for transcription factor
NF-IL6
RL Proc. Natl. Acad. Sci. USA 90:2207-2211 (1993).
RN [6]; RE0006254.
RX MEDLINE; 94052130.
RA Matsusaka
T., Fujikawa K., Nishio Y., Mukaida N., Matsushima K., Kishimoto
RA T., Akira S.
RT Transcription factors NF-IL6 and NF-kappaB
synergistically activate
RT transcription of the inflammatory
cytokines interleukin 6 and interleukin 8
RL Proc. Natl. Acad.
Sci. USA 90:10193-10197 (1993).
RN [7]; RE0004517.
RX
MEDLINE; 92390404.
RA LeClair K. P., Blanar M. A., Sharp P.
A.
RT The p50 subunit of NF-kappaB associates with the NF-IL6
transcription factor
RL Proc. Natl. Acad. Sci. USA 89:8145-8149
(1992).
RN [8]; RE0001943.
RX MEDLINE; 91248826.
RA
Isshiki H., Akira S., Sugita T., Nishio Y., Hashimoto S., Pawlowski
T.,
RA Suematsu S., Kishimoto T.
RT Reciprocal expression
of NF-IL6 and C/EBP in hepatocytes: possible
RT involvement
of NF-IL6 in acute phase protein gene expression
RL New Biol.
3:63-70 (1991).
RN [9]; RE0000469.
RX MEDLINE; 90269225.
RA Akira S., Isshiki H., Sugita T., Tanabe O., Kinoshita S., Nishio
Y.,
RA Nakajima T., Hirano T., Kishimoto T.
RT A nuclear
factor for IL-6 expression (NF-IL6) is a member of a C/EBP family
RL EMBO J. 9:1897-1906 (1990).
RN [10]; RE0003187.
RX
MEDLINE; 90258863.
RA Isshiki H., Akira S., Tanabe O., Nakajima
T., Shimamoto T., Hirano T.,
RA Kishimoto T.
RT Constitutive
and Interleukin-1 (IL-1)-Inducible Factors Interact with the
RT IL-1-Responsive Element in the IL-6 Gene
RL Mol. Cell.
Biol. 10:2757-2764 (1990).
XX