XX
XX
XX
FA GATA-1
XX
SY EF1; EFgammaa; Eryf-1;
GATA-1; GF-1; NF-E1 (1).
XX
OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata; vertebrata; tetrapoda;
mammalia;
OC eutheria; rodentia; myomorpha; muridae; murinae
XX
XX
CL C0003; CC (g); 2.2.1.1.1.1.
XX
SZ 413
AA; 42.6 kDa (cDNA), 50 kDa (SDS)
XX
XX
FT 1 66 transcription
activation domain Act1 [29] [7].
FT 101 193 transcription
activation domain Act2 [29].
FT 198 248 SM00401; ZnF_GATA.
FT 200 239 PF00320; GATA.
FT 204 228 zinc finger 1.
FT
252 302 SM00401; ZnF_GATA.
FT 254 293 PF00320; GATA.
FT
258 282 zinc finger 2.
FT 308 413 transcription activation
domain Act3 [29].
XX
SF transcription of GATA-1 gene in
testis and erythroid cells initiates at
SF different promoters
[2] [17];
SF C-terminal but not N-terminal zinc finger required
for DNA binding [29] and
SF interaction with Sp1
[11];
SF N-terminal zinc finger is largely dispensable in
nonhemapoietic reporter
SF assays but it is strictly required
for terminal erythroid maturation [1]
SF but only partially
for megacaryocytic differentiation [14];
SF replacement of
the two zinc fingers (202-305) by the single zinc finger of
SF areA (658-724) creates a protein which is functional
in induction
SF of globin genes in cultured embryonal stem
cells [13];
SF sequences adjacent to the zinc finger (278-317,
243-304) mediate homo- and
SF heterodimerization (with GATA-2
and -3) [8];
SF large aggregates (foci) have been visualized
at certain nuclear bodies [5];
SF gene maps to the proximal
region of the X chromosome [27];
SF proerythroblasts contain
a transcriptional activator (TAG) of the GATA-1
SF gene whose
activity decreases during terminal differentiation [15];
SF
regulates its own expression [21] [26];
SF while almost all
sequences outside the DNA binding domain are required for
SF full transcriptional activation the first 66 amino acids (Act1)
are most
SF essential [29];
SF translation of GATA-1 mRNA
can start from two different AUG codons giving
SF rise to
the full length 50 kDa protein (GATA-1) or a shorter 40 kDa product
SF (GATA-1s) lacking the amino acids in front of the methionine
at position 84
SF in GATA-1, GATA-1s can hetero-dimerize with
GATA-1 and it has the same
SF DNA-binding activity as GATA-1,
but due to absence of Act1 its ability to
SF activate transcription
is reduced [7];
SF in uninduced MEL cells phosphorylated at
six N-terminal serine residues
SF (26, 49, 72, 142, 178, 187)
after induction with DMSO a 7th serine residue
SF (position
310) gets phosphorylated, but without an effect on its activity
SF [16] [14];
SF the activation domains are mostly dispensable
for megacaryocytic or
SF erythroid differentiation [14] [13].;
XX
CP erythroid and megacaryocytic cells, [3] [1] [17].
EX adrenal gland (right + left),,,adult;none; northern blot; total
RNA; [2].
EX bone marrow,mast cell,,adult;detectable; northern
blot; total RNA; [3].
EX caecum,,,Theiler Stage 22;none; northern
blot; total RNA; [2].
EX caecum,,,Theiler Stage 28;none; northern
blot; total RNA; [2].
EX caecum,,,adult;none; northern blot;
total RNA; [2].
EX cerebellum,,,adult;none; northern blot;
total RNA; [2].
EX cerebrum,,,Theiler Stage 22;none; northern
blot; total RNA; [2].
EX cerebrum,,,Theiler Stage 28;none;
northern blot; total RNA; [2].
EX cerebrum,,,adult;none; northern
blot; total RNA; [2].
EX cerebrum,,,adult;none; northern blot;
total RNA; [2].
EX heart,,,adult;none; northern blot; total
RNA; [2].
EX heart,,,undefined;none; northern blot; total
RNA; [4].
EX kidney (right and left),,,adult;none; northern
blot; total RNA; [4].
EX liver,,,Theiler Stage 20;high; RNA-in
situ hybridization (not further
EX specified); RNA (undefined);
[1].
EX liver,,,adult;none; northern blot; total RNA; [2].
EX lung,,,adult;none; northern blot; total RNA; [2].
EX lung,,,undefined;none;
northern blot; total RNA; [4].
EX mouse, Mus musculus,lymphocyte,,adult;none;
northern blot; total RNA; [2].
EX muscles,myotube,,fetal;none;
northern blot; total RNA; [4].
EX small intestine,,,adult;none;
northern blot; total RNA; [2].
EX spleen,,,adult;detectable;
northern blot; total RNA; [2].
EX stomach,,,undefined;none;
northern blot; total RNA; [4].
EX submandibular gland,,,adult;none;
northern blot; total RNA; [2].
EX testis (right and left),,,Theiler
Stage 22;high; northern blot; total RNA;
EX [2].
EX testis
(right and left),,,Theiler Stage 28;low; northern blot; total
RNA;
EX [2].
EX testis (right and left),,,adult;very low;
northern blot; total RNA; [2].
EX testis (right and left),,,adult;very
low; northern blot; total RNA; [2].
EX thymus,,,adult;none;
northern blot; total RNA; [2].
EX uterus,,,undefined;none;
northern blot; total RNA; [4].
XX
FF required for erythroid
differentiation [24];
FF GATA-1 negative proerythroblasts
undergo developmental arrest and death
FF [18];
FF GATA-2
(mRNA) level is elevated in GATA-1 negative proerythroblasts but
FF does not compensate for GATA-1 deficiency [18];
FF GATA-1
gene expression stimulated by erythropoietin [9] and required
for
FF transcription of other EPO-induced genes [12];
FF synergistic effects of GATA-1 and Sp1 (or EKLF )
(via
FF physical interaction) on gene expression [11];
FF bound and inhibited by estrogen receptor + beta-estradiol
[10] [6];
FF induces the early myeloid cell line 416B to differentiate
into
FF megacaryocytes [20];
FF erythroid differentiation
of murine embryonic stem cells lacking GATA-1 can
FF be rescued
by ectopic expression of chicken GATA-1 , human GATA-3
FF or mouse GATA-4 [13];
XX
IN T01676;
EKLF; mouse, Mus musculus.
IN T00261; ER-alpha; human, Homo
sapiens.
IN T02334; FOG; mouse, Mus musculus.
IN T00305;
GATA-1; mouse, Mus musculus.
IN T01302; GATA-2; mouse, Mus
musculus.
IN T00310; GATA-3; mouse, Mus musculus.
IN T02251;
Lmo2; mouse, Mus musculus.
IN T00752; Sp1; mouse, Mus musculus.
XX
XX
XX
XX
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RT Phosphorylation
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RT Immortalization
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XX