XX
XX
XX
FA Hand1
XX
SY Thing1; Th1; eHand;
Hxt; heart- and neural crest derivatives-expressed 1.
XX
OS mouse, Mus musculus
OC eukaryota; animalia; metazoa; chordata;
vertebrata; tetrapoda; mammalia;
OC eutheria; rodentia; myomorpha;
muridae; murinae
XX
XX
CL C0010; bHLH; 1.2.4.2.5.
XX
SZ 216 AA; 23.8 kDa (cDNA)
XX
XX
SC conceptually
translated from EMBL/GenBank/DDBJ #U21226
XX
FT 9 24 histidine-rich
region (10/16).
FT 94 109 basic region.
FT 95 147 PF00010;
HLH.
FT 100 152 SM00353; HLH.
FT 110 146 helix-loop-helix
domain.
XX
SF bHLH region is 87% identical to that of
Hand2 [2];
SF no homodimerization, DNA-binding depends
on heterodimerization [7];
SF the Hand1-bound half-sites are
highly degenerate [7];
SF Hand1 and Hand2 show a
complementary assymetrical expression in
SF the developing
left and right heart chamber respectively [1];
XX
CP (E10.5:)
portions of the heart, autonomic ganglia, gut, pharyngeal arches;
CP (adult:) intestine, lung, placenta [7]
CN brain, heart,
kidney, lung, skeletal muscle, spleen [7]; F9 embryonal
CN
carcinoma cells [3]
EX adrenal medulla,,,Theiler Stage 21;medium;
RNA-in situ hybridization (not
EX further specified); RNA
(undefined); [2].
EX allantois,,,Theiler Stage 15;none; RNA-in
situ hybridization (not further
EX specified); RNA (undefined);
[3].
EX allantois,,,Theiler Stage 15;none; northern blot;
RNA (undefined); [3].
EX allantois,,,Theiler Stage 15;none;
northern blot; RNA (undefined); [3].
EX allantois,extraembryonic
mesoderm cell,,Theiler Stage 11;low; RNA-in situ
EX hybridization
(not further specified); RNA (undefined); [3].
EX amniotic
ectoderm,extraembryonic mesoderm cell,,Theiler Stage 11;low;
EX RNA-in situ hybridization (not further specified); RNA (undefined);
[3].
EX atrial endocardium,,,Theiler Stage 12;none; RNA-in
situ hybridization (not
EX further specified); RNA (undefined);
[1].
EX atrial endocardium,,,Theiler Stage 15;none; RNA-in
situ hybridization (not
EX further specified); RNA (undefined);
[1].
EX atrial myocardium,,,Theiler Stage 12;detectable; RNA-in
situ hybridization
EX (not further specified); RNA (undefined);
[1].
EX atrial myocardium,,,Theiler Stage 15;detectable; RNA-in
situ hybridization
EX (not further specified); RNA (undefined);
[1].
EX atrio-ventricular canal,,,Theiler Stage 12;detectable;
RNA-in situ
EX hybridization (not further specified); RNA
(undefined); [1].
EX atrio-ventricular canal,,,Theiler Stage
15;detectable; RNA-in situ
EX hybridization (not further specified);
RNA (undefined); [1].
EX atrium of heart (right and left),,,Theiler
Stage 12;none; RNA-in situ
EX hybridization (not further specified);
RNA (undefined); [1].
EX atrium of heart (right and left),,,Theiler
Stage 15;none; RNA-in situ
EX hybridization (not further specified);
RNA (undefined); [1].
EX blastocyst,,,Theiler Stage 4;detectable;
RT-PCR; RNA (undefined); [3].
EX brain,,,adult;high; northern
blot; mRNA (poly-A); [3].
EX brain,,,adult;none; northern
blot; total RNA; [3].
EX caudal region of heart tube,,,Theiler
Stage 12;detectable; RNA-in situ
EX hybridization (not further
specified); RNA (undefined); [1].
EX chorion,,,Theiler Stage
11;none; RNA-in situ hybridization (not further
EX specified);
RNA (undefined); [3].
EX chorion,,,Theiler Stage 15;none;
RNA-in situ hybridization (not further
EX specified); RNA
(undefined); [3].
EX chorion,,,Theiler Stage 15;none; northern
blot; RNA (undefined); [3].
EX chorion,,,Theiler Stage 15;none;
northern blot; RNA (undefined); [3].
EX chorionic ectoderm,extraembryonic
mesoderm cell,,Theiler Stage 11;low;
EX RNA-in situ hybridization
(not further specified); RNA (undefined); [3].
EX compact
myocardial outer layer of ventricle,,,Theiler Stage 15;detectable;
EX RNA-in situ hybridization (not further specified); RNA (undefined);
[1].
EX decidua,,,Theiler Stage 11;high; RNA-in situ hybridization
(not further
EX specified); RNA (undefined); [3].
EX decidua,,,Theiler
Stage 11;none; RNA-in situ hybridization (not further
EX specified);
RNA (undefined); [2].
EX decidua,,,Theiler Stage 11;none;
northern blot; total RNA; [3].
EX dividing egg,,,Theiler Stage
2;detectable; RT-PCR; RNA (undefined); [3].
EX ectoplacental
cone,trophoblast cell,,Theiler Stage 11;high; RNA-in situ
EX hybridization (not further specified); RNA (undefined); [3].
EX endocardial cushion tissue,,,Theiler Stage 15;none; RNA-in
situ
EX hybridization (not further specified); RNA (undefined);
[1].
EX endocardial tissue of left part of ventricle,,,Theiler
Stage 12;none;
EX RNA-in situ hybridization (not further specified);
RNA (undefined); [1].
EX endocardial tissue of left part of
ventricle,,,Theiler Stage 15;none;
EX RNA-in situ hybridization
(not further specified); RNA (undefined); [1].
EX endocardial
tissue of outflow tract,,,Theiler Stage 12;none; RNA-in situ
EX hybridization (not further specified); RNA (undefined); [1].
EX endocardial tissue of outflow tract,,,Theiler Stage 15;none;
RNA-in situ
EX hybridization (not further specified); RNA
(undefined); [1].
EX endocardial tissue of right part of ventricle,,,Theiler
Stage 12;none;
EX RNA-in situ hybridization (not further specified);
RNA (undefined); [1].
EX endocardial tissue of right part
of ventricle,,,Theiler Stage 15;none;
EX RNA-in situ hybridization
(not further specified); RNA (undefined); [1].
EX endocardium
of atrio-ventricular canal,,,Theiler Stage 12;none; RNA-in situ
EX hybridization (not further specified); RNA (undefined); [1].
EX endocardium of atrio-ventricular canal,,,Theiler Stage 15;none;
RNA-in situ
EX hybridization (not further specified); RNA
(undefined); [1].
EX endocardium of inflow tract of heart,,,Theiler
Stage 15;none; RNA-in situ
EX hybridization (not further specified);
RNA (undefined); [1].
EX endocardium of primitive heart tube,,,Theiler
Stage 11;none; RNA-in situ
EX hybridization (not further specified);
RNA (undefined); [1].
EX endocardium of ventriculoinfundibular
fold,,,Theiler Stage 15;none; RNA-in
EX situ hybridization
(not further specified); RNA (undefined); [1].
EX extraembryonic
component,,,Theiler Stage 11;high; RNA-in situ hybridization
EX (not further specified); RNA (undefined); [2].
EX heart,,,Theiler
Stage 21;very low; RNA-in situ hybridization (not further
EX specified); RNA (undefined); [2].
EX heart,,,adult;none;
northern blot; mRNA (poly-A); [3].
EX intestine,,,adult;none;
northern blot; total RNA; [3].
EX kidney (right and left),,,adult;none;
northern blot; mRNA (poly-A); [3].
EX kidney (right and left),,,adult;none;
northern blot; total RNA; [3].
EX lateral plate mesoderm,,,Theiler
Stage 12;detectable; RNA-in situ
EX hybridization (not further
specified); RNA (undefined); [1].
EX lateral plate mesoderm,,,Theiler
Stage 15;detectable; RNA-in situ
EX hybridization (not further
specified); RNA (undefined); [1].
EX left ventricle,,,Theiler
Stage 12;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [1].
EX left ventricle,,,Theiler
Stage 15;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [1].
EX liver,,,adult;none; northern
blot; mRNA (poly-A); [3].
EX liver,,,adult;none; northern
blot; total RNA; [3].
EX lung,,,adult;none; northern blot;
mRNA (poly-A); [3].
EX lung,,,adult;none; northern blot; total
RNA; [3].
EX morula,,,Theiler Stage 3;detectable; RT-PCR;
RNA (undefined); [3].
EX mouse, Mus musculus,,,Theiler Stage
11;high; northern blot; total RNA; [3].
EX mouse, Mus musculus,,,Theiler
Stage 11;none; RNA-in situ hybridization (not
EX further specified);
RNA (undefined); [3].
EX mouse, Mus musculus,,,Theiler Stage
11;very high; northern blot; total RNA;
EX [3].
EX mouse,
Mus musculus,,,Theiler Stage 13;very low; northern blot; total
RNA;
EX [3].
EX mouse, Mus musculus,,,Theiler Stage 14;none;
RNA-in situ hybridization (not
EX further specified); RNA
(undefined); [3].
EX mouse, Mus musculus,,,Theiler Stage 15;very
low; northern blot; total RNA;
EX [3].
EX mouse, Mus musculus,,,Theiler
Stage 17;very low; northern blot; total RNA;
EX [3].
EX
mouse, Mus musculus,undifferentiated embryonic stem cell,,undefined;none;
EX northern blot; RNA (undefined); [3].
EX mural trophoectoderm,trophoblast
cell,,Theiler Stage 15;high; northern
EX blot; RNA (undefined);
[3].
EX muscles,,,adult;high; northern blot; mRNA (poly-A);
[3].
EX myocardium of atrio-ventricular canal,,,Theiler Stage
12;detectable; RNA-in
EX situ hybridization (not further specified);
RNA (undefined); [1].
EX myocardium of atrio-ventricular canal,,,Theiler
Stage 15;detectable; RNA-in
EX situ hybridization (not further
specified); RNA (undefined); [1].
EX myocardium of inflow
tract,,,Theiler Stage 15;detectable; RNA-in situ
EX hybridization
(not further specified); RNA (undefined); [1].
EX myocardium
of left part of ventricle,,,Theiler Stage 12;detectable; RNA-in
EX situ hybridization (not further specified); RNA (undefined);
[1].
EX myocardium of left part of ventricle,,,Theiler Stage
15;detectable; RNA-in
EX situ hybridization (not further specified);
RNA (undefined); [1].
EX myocardium of outflow tract,,,Theiler
Stage 12;detectable; RNA-in situ
EX hybridization (not further
specified); RNA (undefined); [1].
EX myocardium of outflow
tract,,,Theiler Stage 15;detectable; RNA-in situ
EX hybridization
(not further specified); RNA (undefined); [1].
EX myocardium
of primitive heart tube,,,Theiler Stage 11;detectable; RNA-in
EX situ hybridization (not further specified); RNA (undefined);
[1].
EX myocardium of right part of ventricle,,,Theiler Stage
12;detectable; RNA-in
EX situ hybridization (not further specified);
RNA (undefined); [1].
EX myocardium of right part of ventricle,,,Theiler
Stage 15;detectable; RNA-in
EX situ hybridization (not further
specified); RNA (undefined); [1].
EX one-cell egg,,,Theiler
Stage 1;detectable; RT-PCR; RNA (undefined); [3].
EX outflow
tract of heart,,,Theiler Stage 12;detectable; RNA-in situ
EX hybridization (not further specified); RNA (undefined); [1].
EX ovary,,,adult;none; northern blot; total RNA; [3].
EX parietal
yolk sac,trophoblast giant cell,,Theiler Stage 11;high; RNA-in
EX situ hybridization (not further specified); RNA (undefined);
[3].
EX pericardium of primitive heart tube,,,Theiler Stage
11;detectable; RNA-in
EX situ hybridization (not further specified);
RNA (undefined); [1].
EX pericardium of primitive heart tube,,,Theiler
Stage 12;detectable; RNA-in
EX situ hybridization (not further
specified); RNA (undefined); [1].
EX pharyngeal arches,,,Theiler
Stage 15;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [1].
EX placenta,,,Theiler Stage
11;high; northern blot; total RNA; [3].
EX placenta,,,Theiler
Stage 13;very high; northern blot; total RNA; [3].
EX placenta,,,Theiler
Stage 15;very high; northern blot; total RNA; [3].
EX placenta,,,Theiler
Stage 17;very high; northern blot; total RNA; [3].
EX polar
trophectoderm,trophoblast cell,,Theiler Stage 15;high; northern
blot;
EX RNA (undefined); [3].
EX precardiogenic mesoderm,,,Theiler
Stage 11;detectable; RNA-in situ
EX hybridization (not further
specified); RNA (undefined); [1].
EX primitive streak,,,Theiler
Stage 10;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [3].
EX primitive streak,,,Theiler
Stage 11;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [3].
EX primitive streak,,,Theiler
Stage 12;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [3].
EX primitive streak,,,Theiler
Stage 13;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [3].
EX primitive streak,,,Theiler
Stage 14;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [3].
EX primitive streak,,,Theiler
Stage 15;detectable; RNA-in situ hybridization
EX (not further
specified); RNA (undefined); [3].
EX right ventricle,,,Theiler
Stage 12;none; RNA-in situ hybridization (not
EX further specified);
RNA (undefined); [1].
EX right ventricle,,,Theiler Stage 15;none;
RNA-in situ hybridization (not
EX further specified); RNA
(undefined); [1].
EX rostral region of heart tube,,,Theiler
Stage 12;detectable; RNA-in situ
EX hybridization (not further
specified); RNA (undefined); [1].
EX spleen,,,adult;none;
northern blot; mRNA (poly-A); [3].
EX sympathetic trunk,,,Theiler
Stage 21;medium; RNA-in situ hybridization (not
EX further
specified); RNA (undefined); [2].
EX testis (right and left),,,adult;none;
northern blot; mRNA (poly-A); [3].
EX uterus,,,adult;none;
northern blot; total RNA; [3].
EX ventricular myocardium at
the outer curvature,,,Theiler Stage
EX 15;detectable; RNA-in
situ hybridization (not further specified); RNA
EX (undefined);
[1].
XX
FF activator (as heterodimer with E2A-proteins),
may also exert repressor
FF function [7];
FF induces trophoblast
formation in preimplantation embryos while Id-1
FF
inhibits trophoblast differentiation [3];
FF role in left
chamber-specific cell fate decisions and possibly involved in
FF cardiac looping [1] [6];
FF Edn1 (endothelin-1) secretion
stimulates Hand1 expression in the branchial
FF and aortic
arches [4];
FF left-sided expression of Hand1 but not Hand2
is under the control
FF of Nkx2-5 [6];
FF involved in development of segments of the heart tube which
give rise to
FF specific chambers of the heart during cardiogenesis
[5];
XX
IN T01034; Da; fruit fly, Drosophila melanogaster.
IN T00204; E12; human, Homo sapiens.
IN T00207; E47; human,
Homo sapiens.
XX
XX
XX
XX
RN [1]; RE0015953.
RX MEDLINE; 97315821.
RA Srivastava D., Thomas T., Lin Q.,
Kirby M. L., Brown D., Olson E. N.
RT Regulation of cardiac
mesodermal and neural crest development by the bHLH
RT transcription
factor, dHAND
RL Nat. Genet. 16:154-160 (1997).
RN [2];
RE0006279.
RX MEDLINE; 96123273.
RA Srivastava D., Cserjesi
P., Olson E. N.
RT A subclass of bHLH proteins required for
cardiac morphogenesis
RL Science 270:1995-1999 (1995).
RN [3]; RE0015952.
RX MEDLINE; 95401868.
RA Cross J. C.,
Flannery M. L., Blanar M. A., Steingrimsson E., Jenkins N. A.,
RA Copeland N. G., Rutter W. J., Werb Z.
RT Hxt encodes a
basic helix-loop-helix transcription factor that regulates
RT trophoblast cell development
RL Development 121:2513-2523
(1995).
RN [4]; RE0015954.
RX MEDLINE; 98337821.
RA
Thomas T., Kurihara H., Yamagishi H., Kurihara Y., Yazaki Y.,
Olson E. N.,
RA Srivastava D.
RT A signaling cascade involving
endothelin-1, dHAND and Msx1 regulates
RT development of neural-crest-derived
branchial arch mesenchyme
RL Development 125:3005-3014 (1998).
RN [5]; RE0015956.
RX MEDLINE; 98249002.
RA Thomas T.,
Yamagishi H., Overbeek P. A., Olson E. N., Srivastava D.
RT
The bHLH factors, dHAND and eHAND, specify pulmonary and systemic
cardiac
RT ventricles independent of left-right sidedness
RL Dev. Biol. 196:228-236 (1998).
RN [6]; RE0006047.
RX
MEDLINE; 97336070.
RA Biben C., Harvey R. P.
RT Homeodomain
factor Nkx2-5 controls left/right asymmetric expression of bHLH
RT gene eHand during murine heart development
RL Genes Dev.
11:1357-1369 (1997).
RN [7]; RE0003452.
RX MEDLINE; 95311980.
RA Hollenberg S. M., Sternglanz R., Cheng P. F., Weintraub H.
RT Identification of a new family of tissue-specific basic helix-loop-helix
RT proteins with a two-hybrid system
RL Mol. Cell. Biol. 15:3813-3822
(1995).
XX