XX
XX
XX
FA Nkx2-5
XX
SY Csx; Nkx-2.5; cardiac-specific
homeobox protein.
XX
OS mouse, Mus musculus
OC eukaryota;
animalia; metazoa; chordata; vertebrata; tetrapoda; mammalia;
OC eutheria; rodentia; myomorpha; muridae; murinae
XX
XX
HO tinman (Drosophila)
XX
CL C0006; homeo;
3.1.1.15.5.
XX
SZ 318 AA; 34.2 kDa (cDNA)
XX
XX
SC SwissProt #P42582
XX
FT 10 19 conserved decapeptide.
FT 46 121 trans-activating domain [7].
FT 98 135 charged region
(10-,10+/39).
FT 136 196 homeo domain.
FT 137 199 SM00389;
HOX.
FT 138 194 PF00046; homeobox.
FT 203 318 inhibitory
domain [7].
FT 210 226 NK2-specific domain.
XX
SF
at least two different consensus binding sequences [7];
SF
the potent trans-activation domain is maked by the alanine- and
SF proline-rich C-terminus [7];
XX
CP (cell lines). Fibroblast:
NIH3T3; Bone marrow stroma: BAd; Thymic stroma:
CP SCL-19;
Lymph node stroma: TSL-1 [1]
CN (cell lines): Fibroblasts:
Balb/c 3T3, STO; Myoblasts: 3H10T1/2, C2C12;
CN Myocytes:
C3H10T1/2, C2C12; Bone marrow stroma: A6. BMS; Thymic stroma:
CN B6T-ea, SCL-5,SCL-13; Lymph node stroma: TSL-3 [1]
EX atrium
of heart (right and left),,,Theiler Stage 21;detectable; RNA-in
situ
EX hybridization (radioactive); RNA (undefined); [1].
EX bone marrow,,,adult;none; RNAse protection assay; total RNA;
[1].
EX brain,,,Theiler Stage 28;none; RNAse protection assay;
total RNA; [1].
EX brain,,,adult;none; RNAse protection assay;
total RNA; [1].
EX brain,,,fetal;none; RNAse protection assay;
total RNA; [1].
EX dorsal aorta,,,Theiler Stage 14;none; RNA-in
situ hybridization
EX (radioactive); RNA (undefined); [1].
EX endocardial cushion tissue,,,Theiler Stage 21;none; RNA-in
situ
EX hybridization (radioactive); RNA (undefined); [1].
EX endoderm of foregut,,,Theiler Stage 12;detectable; RNA-in situ
EX hybridization (radioactive); RNA (undefined); [1].
EX heart,,,Theiler
Stage 19;detectable; RNA-in situ hybridization
EX (radioactive);
RNA (undefined); [1].
EX heart,,,adult;high; RNAse protection
assay; total RNA; [1].
EX heart,,,fetal;very high; RNAse protection
assay; total RNA; [1].
EX heart,myogenic cell,,Theiler Stage
13;detectable; RNA-in situ hybridization
EX (radioactive);
RNA (undefined); [1].
EX kidney (right and left),,,adult;none;
RNAse protection assay; total RNA;
EX [1].
EX large intestine,,,adult;none;
RNAse protection assay; total RNA; [1].
EX liver,,,adult;none;
RNAse protection assay; total RNA; [1].
EX liver,,,fetal;none;
RNAse protection assay; total RNA; [1].
EX lung,,,adult;none;
RNAse protection assay; total RNA; [1].
EX lymph node,,,adult;none;
RNAse protection assay; total RNA; [1].
EX muscles,,,adult;none;
RNAse protection assay; total RNA; [1].
EX ovary,,,adult;none;
RNAse protection assay; total RNA; [1].
EX pericardium,,,Theiler
Stage 21;none; RNA-in situ hybridization
EX (radioactive);
RNA (undefined); [1].
EX primitive heart tube,,,Theiler Stage
13;detectable; RNA-in situ
EX hybridization (radioactive);
RNA (undefined); [1].
EX roof of pharynx,,,Theiler Stage 15;none;
RNA-in situ hybridization
EX (radioactive); RNA (undefined);
[1].
EX small intestine,,,adult;none; RNAse protection assay;
total RNA; [1].
EX splanchnopleure,,,Theiler Stage 12;detectable;
RNA-in situ hybridization
EX (radioactive); RNA (undefined);
[1].
EX spleen,,,adult;low; RNAse protection assay; total
RNA; [1].
EX stomach,,,adult;none; RNAse protection assay;
total RNA; [1].
EX testis (right and left),,,adult;none; RNAse
protection assay; total RNA;
EX [1].
EX thymus,,,adult;none;
RNAse protection assay; total RNA; [1].
EX thyroid gland,,,Theiler
Stage 19;detectable; RNA-in situ hybridization
EX (radioactive);
RNA (undefined); [1].
EX tongue,,,adult;low; RNAse protection
assay; total RNA; [1].
EX uterus,,,adult;none; RNAse protection
assay; total RNA; [1].
EX venous sinus of primitive heart,,,Theiler
Stage 14;high; RNA-in situ
EX hybridization (radioactive);
RNA (undefined); [1].
EX venous sinus of primitive heart,,,Theiler
Stage 15;detectable; RNA-in situ
EX hybridization (radioactive);
RNA (undefined); [1].
EX ventricle of heart (right and left),,,Theiler
Stage 21;detectable; RNA-in
EX situ hybridization (radioactive);
RNA (undefined); [1].
XX
FF modest activator [7];
FF probably important for myocard development [1];
FF expression
first detected in myocardiogenic progenitor cells during early
FF headfold stages, then maintained in cardiomyocytes of embryonic,
fetal and
FF adult heart [1];
FF also expressed in pharyngeal
endoderm precursors, later in pharyngeal floor;
FF finally,
expression is restricted to the thyroid primordium [1];
FF
activates with SRF cardiac alpha-actin transcription [6];
FF induces left-sided expression of Hand1 but not Hand2
[5];
FF directly or indirectly autoregulated by negative
feedback [3];
FF transcriptional activator, Nkx2-5 and HMX1
act as transcriptional
FF antagonists [4];
XX
XX
XX
XX
RN [1]; RE0003810.
RX MEDLINE; 94116443.
RA Lints T. J., Parsons L. M., Hartley L., Lyons I., Harvey R.
P.
RT Nkx-2.5: a novel murine homeobox gene expressed in early
heart progenitor
RT cells and their myogenic descendants
RL Development 119:419-431 (1993).
RN [2]; RE0015782.
RX MEDLINE; 11042197.
RA Kasahara H., Usheva A., Ueyama T.,
Aoki H., Horikoshi N., Izumo S.
RT Characterization of homo-
and heterodimerization of cardiac Csx/Nkx2.5
RT homeoprotein
RL J. Biol. Chem. 276:4570-4580 (2001).
RN [3]; RE0015753.
RX MEDLINE; 99169087.
RA Tanaka M., Wechsler S. B., Lee I.
W., Yamasaki N., Lawitts J. A., Izumo S.
RT Complex modular
cis-acting elements regulate expression of the cardiac
RT
specifying homeobox gene Csx/Nkx2.5
RL Development 126:1439-1450
(1999).
RN [4]; RE0016078.
RX MEDLINE; 99223479.
RA
Amendt B. A., Sutherland L. B., Russo A. F.
RT Transcriptional
antagonism between Hmx1 and Nkx2.5 for a shared DNA-binding
RT site
RL J. Biol. Chem. 274:11635-11642 (1999).
RN [5];
RE0006047.
RX MEDLINE; 97336070.
RA Biben C., Harvey R.
P.
RT Homeodomain factor Nkx2-5 controls left/right asymmetric
expression of bHLH
RT gene eHand during murine heart development
RL Genes Dev. 11:1357-1369 (1997).
RN [6]; RE0015484.
RX MEDLINE; 97042466.
RA Chen C.Y., Schwartz R.J.
RT Recruitment
of the tinman homolog Nkx-2.5 by serum response factor
RT
activates cardiac alpha-actin gene transcription
RL Mol. Cell.
Biol. 16:6372-6384 (1996).
RN [7]; RE0003808.
RX MEDLINE;
95318148.
RA Chen C. Y., Schwartz R. J.
RT Identification
of novel DNA binding targets and regulatory domains of a
RT
murine tinman homeodomain factor, nkx-2.5
RL J. Biol. Chem.
270:15628-15633 (1995).
RN [8]; RE0003811.
RX MEDLINE;
94244488.
RA Lints T. J., Parsons L. M., Hartley L., Lyons
I., Harvey R. P.
RT Nkx-2.5: a novel murine homeobox gene
expressed in early heart progenitor
RT cells and their myogenic
descendants (erratum)
RL Development 119:969-969 (1993).
RN [9]; RE0003812.
RX MEDLINE; 93376760.
RA Komuro I.,
Izumo S.
RT Csx: A murine homeobox-containing gene specifically
expressed in the
RT developing heart
RL Proc. Natl. Acad.
Sci. USA 90:8145-8149 (1993).
XX