XX
XX
XX
FA Pbx-1a
XX
SY Pbx-1; Pbx1; pre
B-cell leukemia transcription factor 1a; Prl.
XX
OS human,
Homo sapiens
OC eukaryota; animalia; metazoa; chordata; vertebrata;
tetrapoda; mammalia;
OC eutheria; primates
XX
XX
HO Exd (Drosophila), MATa1 (yeast), ceh-20 (C.elegans)
XX
CL C0006; homeo; 3.1.1.18.4.1.
XX
SZ 430 AA; 46.6 kDa
(cDNA)
XX
XX
SC SwissProt #P40424
XX
FT 1
140 PKNOX1 interaction domain [4].
FT 33 232 PF03792;
PBX.
FT 127 135 alanine-rich region (9/9).
FT 233 298
SM00389; HOX.
FT 234 293 PF00046; homeobox.
FT 236 295
homeo domain.
FT 334 430 replaced by 334-347 in Pbx-1b.
FT 347 430 transactivation domain [5].
XX
SF divergent
homeo domain, most similar to yeast MATa1 [1];
SF TALE (three
amino acid loop extension) homeo domain superclass [6];
SF
co-factor for many, but not all homeo domain proteins [8] [9];
SF cooperative binding with Meis-1 and PKNOX1
which
SF is disrupted in the oncoprotein E2A-Pbx1
[7] [4];
SF subunit of the UEF-3 complex [3];
SF interacts DNA indepently with PKNOX1 and increases
binding
SF affinity [3] [4];
SF PKNOX1-Pbx-1 complexes
are present during mouse embryogenesis [4];
SF mediates DNA
dependent ternary complex with HOXB1 [5] and PKNOX1
SF [4];
XX
CN (cell lines:) lymphoid cells
EX adrenal gland (right and left),,,fetal;high; northern blot;
mRNA (poly-A);
EX [1].
EX blood,basophil granulocyte,Circulatory
System & Hematopoietic
EX System,adult;low; northern blot;
mRNA (poly-A); [1].
EX blood,eosinophil granulocyte,Circulatory
System & Hematopoietic
EX System,adult;low; northern blot;
mRNA (poly-A); [1].
EX blood,lymphocyte,Circulatory System
& Hematopoietic System,adult;low;
EX northern blot; mRNA (poly-A);
[1].
EX blood,monocyte,Circulatory System & Hematopoietic
System,adult;low;
EX northern blot; mRNA (poly-A); [1].
EX blood,neutrophil granulocyte,Circulatory System & Hematopoietic
EX System,adult;low; northern blot; mRNA (poly-A); [1].
EX
brain,,,fetal;very high; northern blot; mRNA (poly-A); [1].
EX gut,,,fetal;medium; northern blot; mRNA (poly-A); [1].
EX heart,,,adult;medium; northern blot; mRNA (poly-A); [1].
EX heart,,,fetal;high; northern blot; mRNA (poly-A); [1].
EX kidney (right and left),,,adult;none; northern blot; mRNA (poly-A);
[1].
EX kidney (right and left),,,fetal;high; northern blot;
mRNA (poly-A); [1].
EX liver,,,fetal;medium; northern blot;
mRNA (poly-A); [1].
EX lung (right and left),,,fetal;high;
northern blot; mRNA (poly-A); [1].
EX ovary (right and left),,,adult;medium;
northern blot; mRNA (poly-A); [1].
EX spleen,,,fetal;medium;
northern blot; mRNA (poly-A); [1].
EX thymus,,,adult;none;
northern blot; mRNA (poly-A); [1].
EX thymus,,,fetal;medium;
northern blot; mRNA (poly-A); [1].
EX tonsil,,,adult;medium;
northern blot; mRNA (poly-A); [1].
EX uterus,,,adult;medium;
northern blot; mRNA (poly-A); [1].
XX
FF in acute lymphoblastic
leukemias, a t(1;
FF 19)(q23;
FF p13.3) translocation
fuses the trans-activation domain encoded by the E2A
FF gene
with the homeobox of PBX1 [15] [16] [14];
FF the resulting
chimeric protein (E2A-Pbx1) causes acute myeloid leukemia
FF [12] and induces enhanced proliferation as well as apoptosis
[13] probably
FF by interfering with differentiation regulation
by homeo domain factors [8]
FF [9] and acting as a strong
activator [10];
FF acts as cofactor of HOXB1 and
cooperative transcriptional
FF activator [5];
FF PKNOX2/Pbx-1a
heterodimer (T01481/T05155) shows faster DNA-dissociation
FF rate (binding site: ) than PKNOX1/Pbx-1a heterodimer
FF (T01481/T04122) [2];
XX
IN T04122; PKNOX1; human, Homo
sapiens.
IN T05155; PKNOX2; human, Homo sapiens.
XX
XX
XX
XX
RN [1]; RE0005181.
RX MEDLINE; 92049345.
RA Monica K., Saltman D., Nourse J., Galili N., Cleary M. L.
RT PBX2 and PBX3, new homeobox genes with extensive homology to
the human
RT proto-oncogene PBX1
RL Mol. Cell. Biol. 11:6149-6157
(1991).
RN [2]; RE0017883.
RX MEDLINE; 21970090.
RA
Fognani C., Kilstrup-Nielsen C., Berthelsen J., Ferretti E., Zappavigna
V.,
RA Blasi F.
RT Characterization of PREP2, a paralog
of PREP1, which defines a novel
RT sub-family of the MEINOX
TALE homeodomain transcription factors
RL Nucleic Acids Res
30:2043-2051 (2002).
RN [3]; RE0015488.
RX MEDLINE; 98151366.
RA Berthelsen J., Zappavigna V., Mavilio F., Blasi F.
RT Prep1,
a novel functional partner of Pbx proteins
RL EMBO J. 17:1423-1433
(1998).
RN [4]; RE0015500.
RX MEDLINE; 98151367.
RA
Berthelsen J., Zappavigna V., Ferretti E., Mavilio F., Blasi F.
RT The novel homeoprotein Prep1 modulates Pbx-Hox protein cooperativity
RL EMBO J. 17:1434-1445 (1998).
RN [5]; RE0005973.
RX
MEDLINE; 97361844.
RA Rocco di G., Mavilio F., Zappavigna
V.
RT Functional dissection of a transcripitonally active,
target-specific
RT Hox-Pbx complex
RL EMBO J. 16:3644-3654
(1997).
RN [6]; RE0014544.
RX MEDLINE; 97478541.
RA
Burglin T. R.
RT Analysis of TALE superclass homeobox genes
(MEIS, PBC, KNOX, Iroquois,
RT TGIF) reveals a novel domain
conserved between plants and animals
RL Nucleic Acids Res.
25:4173-4180 (1997).
RN [7]; RE0015495.
RX MEDLINE; 98070790.
RA Knoepfler P. S., Calvo K. R., Chen H., Antonarakis S. E., Kamps
M. P.
RT Meis1 and pKnox1 bind DNA cooperatively with Pbx1
utilizing an interaction
RT surface disrupted in oncoprotein
E2a-Pbx1
RL Proc. Natl. Acad. Sci. USA 94:14553-14558 (1997).
RN [8]; RE0005178.
RX MEDLINE; 95311977.
RA Lu Q., Knoepfler
P. S., Scheele J., Wright D. D., Kamps M. P.
RT Both Pbx1
and E2A-Pbx1 bind the DNA motif ATCAATCAA cooperatively with the
RT products of multiple murine Hox genes, some of wich are themselves
oncogenes
RL Mol. Cell. Biol. 15:3786-3795 (1995).
RN
[9]; RE0005179.
RX MEDLINE; 95349565.
RA Phelan M. L.,
Rambaldi I., Featherstone M. S.
RT Cooperative interactions
between HOX and PBX proteins mediated by a
RT conserved peptide
motif
RL Mol. Cell. Biol. 15:3989-3997 (1995).
RN [10];
RE0003009.
RX MEDLINE; 94254851.
RA Lu Q., Wright D. D.,
Kamps M. P.
RT Fusion with E2A converts the Pbx1 homeodomain
protein into a constitutive
RT transcriptional activator in
human leukemias carrying the t(1;19)
RT translocation
RL Mol. Cell. Biol. 14:3938-3948 (1994).
RN [11]; RE0005177.
RX MEDLINE; 94308119.
RA Kagawa N., Ogo A., Takahashi Y.,
Iwamatsu A., Waterman M. R.
RT A cAMP-regulatory sequence
(CRS1) of CYP17 is a cellular target for the
RT homeodomain
protein Pbx1
RL J. Biol. Chem. 269:18716-18719 (1994).
RN [12]; RE0004018.
RX MEDLINE; 93109319.
RA Kamps M.
P., Baltimore D.
RT E2A-Pbx1, the t(1;19) translocation protein
of human pre-B-cell acute
RT lymphocytic leukemia causes acute
myeloid leukemia in mice
RL Mol. Cell. Biol. 13:351-357 (1993).
RN [13]; RE0005180.
RX MEDLINE; 93386756.
RA Dedera D.
A., Waller E. K., LeBrun D. P., Sen-Majumdar A., Stevens M. E.,
RA Barsh G. S., Cleary M. L.
RT Chimeric homeobox gene E2A-PBX1
induces proliferation, apoptosis, and
RT malignant lymphomas
in transgenic mice
RL Cell 74:833-843 (1993).
RN [14];
RE0000671.
RX MEDLINE; 91160969.
RA Kamps M. P., Look
A. T., Baltimore D.
RT The human t(1;19) translocation in
pre-B ALL produces multiple nuclear
RT E2A-Pbx1 fusion proteins
with differing transforming potentials
RL Genes Dev. 5:358-368
(1991).
RN [15]; RE0000161.
RX MEDLINE; 90150281.
RA Nourse J., Mellentin J. D., Galili N., Wilkinson J., Stanbridge
E., Smith
RA S. D., Cleary M. L.
RT Chromosomal translocation
t(1;19) results in synthesis of a homeobox fusion
RT mRNA
that codes for a potential chimeric transcription factor
RL
Cell 60:535-545 (1990).
RN [16]; RE0004014.
RX MEDLINE;
90150282.
RA Kamps M. P., Murre C., Sun X. H., Baltimore D.
RT A new homeobox gene contributes the DNA binding domain oft
the t(1; 19)
RT translocation protein in pre-B ALL
RL
Cell 60:547-555 (1990).
XX