XX
 XX
 XX
 FA E2F-1
 XX
 SY RBB3.
 XX
 OS
human, Homo sapiens
 OC eukaryota; animalia; metazoa; chordata;
vertebrata; tetrapoda; mammalia;
 OC eutheria; primates

XX
 XX
 CL C0023; fork head; 3.3.3.1.1.
 XX
 SZ 437
AA; 46.9 kDa (cDNA), 54 kDa (SDS) [21] [20], 60 kDa (SDS) [17]
[18]
 XX
 XX
 SC SwissProt #Q01094
 XX
 FT 1 124
cyclin A/cdk2 binding.
 FT 89 184 DNA-binding domain.
 FT
109 127 basic region.
 FT 123 338 PF02319; E2F_TDP.
 FT
128 181 potential helix-loop-helix domain.
 FT 128 142 helix
I.
 FT 150 191 dimerization domain.
 FT 156 181 helix II.

FT 199 234 potential leucine zipper (VL3CL).
 FT 368 437 transcription
activating region.
 FT 409 426 Rb-binding region.
 XX

SF homo- and heterodimerizes, heterodimers with DP-1 (= E2F activity)
show
 SF enhanced DNA- and Rb-binding;
 SF heterodimerization
with DP-1 depends on an intact zipper motif [16];
 SF prior
to crystal structure belongs to bHLH-zip factors [2];
 SF also
binds cyclinA/cdk2, but not the individual subunits [5];
 SF
the Rb-binding region also contacts TBP [15];
 XX
 FF E2F-1
contributes the transcriptional activation domain to E2F-1/DP-1

FF heterodimers (E2F) [17] [18];
 FF overexpression may cause
neoplastic transformation [8];
 FF deregulated function in
cooperation with ras causes oncogenic
 FF transformation [14];

FF autoregulates its own promoter in a cell cycle-dependent manner
[6] [10]
 FF [12];
 FF binding of Rb masks the transcription
activation region and prevents it
 FF from interacting with
TBP [15];
 FF phosphorylation at Ser-332 and Ser-337 at the
end of G1 phase, presumably
 FF by cdc2 or cdk2, inhibits Rb
binding [7], inhibits DNA-binding by a
 FF E2F-1/cyclin A/cdk2
complex [5] [9] [13], largely enhances interaction with
 FF
adenoviral E4 [7];
 FF no phosphorylation by cyclin E/cdk2
[9];
 FF expression of E2F-1 activity is lost upon senescence
of cells [11];
 FF E2F-1 regulates the expression of Apaf-1,
the gene for apoptosis
 FF protease-activating factor 1;

FF provides a direct link between the deregulation of the pRB
pathway and
 FF apoptosis [1];
 FF expression is up-regulated
in G1/S phase transition and during S phase in
 FF T-cells,
where E2F-1 is predominantly present in a free form without bound

FF regulatory protein (pRb) [4];
 XX
 IN T03240; DDB; human,
Homo sapiens.
 IN T01548; DP-1; human, Homo sapiens.
 IN
T01549; DP-1; mouse, Mus musculus.
 IN T03000; DP-2; human,
Homo sapiens.
 IN T01542; E2F-1; human, Homo sapiens.
 IN
T01544; E2F-2; human, Homo sapiens.
 IN T00671; p53; human,
Homo sapiens.
 IN T00722; pRb; human, Homo sapiens.
 IN
T00794; TBP; human, Homo sapiens.
 XX
 XX
 XX
 XX

RN [1]; RE0016344.
 RX MEDLINE; 21283226.
 RA Moroni M.
C., Hickman E. S., Denchi E. L., Caprara G., Colli E., Cecconi

RA F., Muller H., Helin K.
 RT Apaf-1 is a transcriptional
target for E2F and p53.
 RL Nat. Cell Biol. 3:552-558 (2001).

RN [2]; RE0015322.
 RX MEDLINE; 99190701.
 RA Zheng N.,
Fraenkel E., Pabo C. O., Pavletich N. P.
 RT Structural basis
of DNA recognition by the heterodimeric cell cycle
 RT transcription
factor E2F-DP
 RL Genes Dev. 13:666-674 (1999).
 RN [3];
RE0014167.
 RX MEDLINE; 98078680.
 RA Hayes S., Shiyanov
P., Chen X., Raychaudhuri P.
 RT DDB, a putative DNA repair
protein, can function as a transcriptional
 RT partner of E2F1

RL Mol. Biol. Cell 18:240-249 (1998).
 RN [4]; RE0012605.

RX MEDLINE; 96239498.
 RA Moberg K., Starz M. A., Lees J. A.

RT E2F-4 switches from p130 to p107 and pRB in response to cell
cycle reentry
 RL Mol. Cell. Biol. 16:1436-1449 (1996).

RN [5]; RE0003299.
 RX MEDLINE; 95059071.
 RA Xu M., Sheppard
K. A., Peng C.-Y., Yee A. S., Piwnica-Worms H.
 RT Cyclin A/CDK2
binds directly to E2F-1 and inhibits the DNA-binding activity

RT of E2F-1/DP-1 by phosphorylation
 RL Mol. Cell. Biol. 14:8420-8431
(1994).
 RN [6]; RE0003306.
 RX MEDLINE; 95021188.
 RA
Neuman E., Flemington E. K., Sellers W. R., Kaelin jr W. G.

RT Transcription of the E2F-1 gene is rendered cell cycle dependent
by E2F
 RT DNA-binding sites within its promoter
 RL Mol.
Cell. Biol. 14:6607-6615 (1994).
 RN [7]; RE0003308.
 RX
MEDLINE; 94373816.
 RA Fagan R., Flint K. J., Jones N.

RT Phosphorylation of E2F-1 modulates its interaction with the
retinoblastoma
 RT gene product and the adenoviral E4 19 kDa
protein
 RL Cell 78:799-811 (1994).
 RN [8]; RE0003309.

RX MEDLINE; 94320597.
 RA Singh P., Wong S. H., Hong W.

RT Overexpression of E2F-1 in rat embryo fibroblasts leads to
neoplastic
 RT transformation
 RL EMBO J. 13:3329-3338 (1994).

RN [9]; RE0003310.
 RX MEDLINE; 95047332.
 RA Dynlacht B.
D., Flores O., Lees J. A., Harlow E.
 RT Differential regulation
of E2F trans-activation by cyclin/cdk2 complexes
 RL Genes
Dev. 8:1772-1786 (1994).
 RN [10]; RE0003311.
 RX MEDLINE;
95047312.
 RA Hsiao K. M., McMahon S. L., Farnham P. J.

RT Multiple DNA elements are required for the growth regulation
of the mouse
 RT E2F1 promoter
 RL Genes Dev. 8:1526-1537
(1994).
 RN [11]; RE0003312.
 RX MEDLINE; 94266806.

RA Dimri G. P., Hara E., Campisi J.
 RT Regulation of two E2F-related
genes in presenescent and senescent human
 RT fibroblasts

RL J. Biol. Chem. 269:16180-16186 (1994).
 RN [12]; RE0003313.

RX MEDLINE; 95047311.
 RA Johnson D. G., Ohtani K., Nevins
J. R.
 RT Autoregulatory control of E2F1 expression in response
to positive and
 RT negative regulators of cell cycle progression

RL Genes Dev. 8:1514-1525 (1994).
 RN [13]; RE0003314.

RX MEDLINE; 94306513.
 RA Krek W., Ewen M. E., Shirodkar S.,
Arany Z., Kaelin jr W. G., Livingston D.
 RA M.
 RT Negative
regulation of the growth-promoting transcription factor E2F-1
by a
 RT stably bound cyclin A-dependent protein kinase

RL Cell 78:161-172 (1994).
 RN [14]; RE0003545.
 RX MEDLINE;
95108050.
 RA Johnson D. G., Cress W. D., Jakoi L., Nevins
J. R.
 RT Oncogenic capacity of the E2F1 gene
 RL Proc.
Natl. Acad. Sci. USA 91:12823-12827 (1994).
 RN [15]; RE0003294.

RX MEDLINE; 94077697.
 RA Hagemeier C., Cook A., Kouzarides
T.
 RT The retinoblastoma protein binds E2F residues required
for activation in
 RT vivo and TBP binding in vitro
 RL
Nucleic Acids Res. 21:4998-5004 (1993).
 RN [16]; RE0003300.

RX MEDLINE; 94069342.
 RA Krek W., Livingston D. M., Shirodkar
S.
 RT Binding to DNA and the retinoblastoma gene product promoted
by complex
 RT formation of different E2F family members

RL Science 262:1557-1560 (1993).
 RN [17]; RE0003281.
 RX
MEDLINE; 92346721.
 RA Kaelin W. G., Krek W., Sellers W. R.,
DeCaprio J. A., Ajchenbaum F., fuchs
 RA C. S., Chittenden
T., Blanar M. A., Livingston D. M., Flemington E. K.
 RT Expression
cloning of a cDNA encoding a retinoblastoma-binding protein with

RT E2F-like properties
 RL Cell 70:351-364 (1992).
 RN [18];
RE0003282.
 RX MEDLINE; 92346720.
 RA Helin K., Lees J.
A., Vidal M., Dyson N., Fattaey A.
 RT A cDNA encoding a pRB-binding
protein with properties of the transcription
 RT factor E2F

RL Cell 70:337-350 (1992).
 RN [19]; RE0003284.
 RX MEDLINE;
93078763.
 RA Shan B., Zhu X., Chen P.-L., Durfee T., Yang
Y., Sharp D., Lee W.-H.
 RT Molecular cloning of cellular genes
encoding retinoblastoma-associated
 RT proteins: identification
of a gene with properties of the transcription
 RT factor E2F

RL Mol. Cell. Biol. 12:5620-5631 (1992).
 RN [20]; RE0000163.

RX MEDLINE; 91256306.
 RA Chellappan S. P., Hiebert S., Mudryj
M., Horowitz J. M., Nevins J. R.
 RT The E2F transcription
factor is a cellular target for the RB protein
 RL Cell 65:1053-1061
(1991).
 RN [21]; RE0001236.
 RX MEDLINE; 89219051.

RA Yee A. S., Raychaudhuri P., Jakoi L., Nevins J. R.
 RT The
Adenovirus-Inducible Factor E2F Stimulates Transcription after
Specific
 RT DNA Binding
 RL Mol. Cell. Biol. 9:578-585
(1989).
 XX